We narrowed to 71,484 results for: nin

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Dual-CRISPR-Cas9-U6-H1 (pBP48)

Plasmid

#218932

Purpose

The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

Depositor

Baoxu Pang

Article

Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

Type

Empty backbone

Use

CRISPR

Tags

2A-Puro and 3XHA

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH-YE1-BE4max

Plasmid

#198549

Purpose

Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-GCaMP6f

Plasmid

#221039

Purpose

Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

Insert

GCaMP6f

Use

AAV; Rat targeting

Expression

Mammalian

Mutation

A317E

Promoter

Rat MCH

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA

Plasmid

#156449

Purpose

For transient expression of human PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5A (PDS5A Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-Ce67

Plasmid

#124680

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xFYVE_hrs

Plasmid

#67663

Purpose

Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-2xFYVE

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2-mCherry

Plasmid

#140571

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-ABE8.17-m+V106W

Plasmid

#198534

Purpose

Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-ABE8.17-m+V106W

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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ZNF217_pLX307

Plasmid

#98384

Purpose

Lentiviral expression of ZNF217

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ZNF217 (ZNF217 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBeta-MHC-promoter DV (JDW 26)

Plasmid

#229835

Purpose

A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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HT110_FCMV_QuasAr6b_citrine

Plasmid

#178823

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Lentiviral

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-E2

Plasmid

#194590

Purpose

Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

E2 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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rAAV-U6-MeCP2shRNA-CamKIIa-mCherry

Plasmid

#169704

Purpose

MeCP2 Knockdown

Depositor

Ana Oliveira

Article

Gulmez Karaca et al Neurobiol Learn Mem. 2018 Mar;149:84-97.

Insert

shRNA against MeCp2 (Mecp2 Mouse)

Use

AAV

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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P25-pZE-SUMO-PbTTA

Plasmid

#204634

Purpose

ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

Depositor

Aditya Kunjapur

Article

Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

Insert

His-SUMO-TEV-PbTTA

Use

Synthetic Biology

Tags

His Tag; SUMO Tag; TEV Protease Site

Expression

Bacterial

Promoter

PLtetO-1 promoter

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

Plasmid

#228726

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

Plasmid

#228725

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

Plasmid

#228727

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

Plasmid

#228728

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

Plasmid

#228730

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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