We narrowed to 17,014 results for: EMB

1. pMS33: pTwinStrep-SUMO-PmcTnsD

   Plasmid

   #168166

   Purpose

   PmcTnsD expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsD
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TAL2274

   Plasmid

   #36711

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MDM2-TALEN_Left (Mdm2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pR540

   Plasmid

   #207885

   Purpose

   template for 2 gRNA amplification

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   scaffold-pU6
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS021

   Plasmid

   #207917

   Purpose

   Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKE013

   Plasmid

   #207916

   Purpose

   Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-2-Zfp36-3'UTR

   Plasmid

   #61780

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Zfp36

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Zfp36 3'UTR
   

   Use

   Luciferase

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET15b+PCNA1 P186C

   Plasmid

   #66173

   Purpose

   Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   P186C

   Promoter

   T7 promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTPI1-EMC1-GFP-APEX2-URA3

   Plasmid

   #184776

   Purpose

   Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   APEX2
   

   Expression

   Yeast

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. C514-E47: attB5r-Halotag-nostop-attB1r

   Plasmid

   #162963

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Halotag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-INPP5J

    Plasmid

    #171949

    Purpose

    Expresses INPP5J in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP5J (INPP5J Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P17

    Plasmid

    #120028

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mEos4b
    

    Use

    Pcr template

    Tags

    mEos4b

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5xFbx15 (-544/-527)-TK-Luc

    Plasmid

    #15686

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    5 x Fbx15 enhancer (Oct/Sox binding site)
    

    Use

    Luciferase

    Mutation

    Wild-type

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

13. mRuby2-AKTIP

    Plasmid

    #171942

    Purpose

    Expresses AKTIP in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    AKTIP (AKTIP Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTF3-AK (clone3)

    Plasmid

    #21165

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    POUF5F1 (genomic DNA) (POU5F1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Mutation

    NeoR, EGFP, and SV40 polyA are between the two ge…

    Available Since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS/KS-mId2sp

    Plasmid

    #16044

    Depositor

    Robert Benezra

    Article

    Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

    Insert

    Id2 (Id2 Mouse)
    

    Expression

    Bacterial

    Mutation

    cDNA nucleotides 1-195

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pMaCTag-Z06

    Plasmid

    #120049

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    sfGFP
    

    Use

    Pcr template

    Tags

    sfGFP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-puro-eGFP-HEC1-9A

    Plasmid

    #114023

    Purpose

    Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
    

    Expression

    Mammalian

    Mutation

    Hec1 - 9A

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCG-D-mSpyCatcher-His6-E

    Plasmid

    #87376

    Purpose

    An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    mSpyCatcher
    

    Use

    Synthetic Biology

    Tags

    His6 tag

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMC-Ura-Cen-lvl1

    Plasmid

    #176182

    Purpose

    preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAL725

    Plasmid

    #119862

    Purpose

    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    Leucine 244 to Cysteine, Aspartate 287 to Cysteine

    Promoter

    T5

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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