We narrowed to 26,138 results for: Vit

1. pcDNA3-Lyso-ExRai-AktAR2

   Plasmid

   #184052

   Purpose

   Lysosome-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-ExRai-AktAR2
   

   Use

   Tags

   Lysosome-associated membrane protein 1 (LAMP1).

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN3P-NLS-EGFP-deadBtgh-3NLS

   Plasmid

   #194470

   Purpose

   Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

   Depositor

   Mathieu Boulard

   Article

   Formichetti et al Dev Cell 10 Jan 2024

   Insert

   BtGH84
   

   Use

   For in vitro transcription from t3 promoter after…

   Tags

   SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

   Expression

   Mutation

   Aspartic Acid 242 (in Dennis et al.) changed to A…

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sumo3 PolQM1

   Plasmid

   #78462

   Purpose

   Inducible expression in E. coli

   Depositor

   Susan Wallace, Sylvie Doublie

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; Q2513R (please see depositor commen…

   Promoter

   T7

   Available since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pEYFP-hDF

   Plasmid

   #72584

   Purpose

   human dysferlin full length tagged with YFP

   Depositor

   Renzhi Han

   Article

   Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

   Insert

   dysferlin (DYSF Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. HypaCas9

   Plasmid

   #138557

   Purpose

   Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   HypaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-GRK6

   Plasmid

   #32693

   Depositor

   Robert Lefkowitz

   Article

   Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

   Insert

   GRK6 (GRK6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUS_anion

   Plasmid

   #98656

   Purpose

   Expresses untagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hsyn-GRAB_Ado1.0med

   Plasmid

   #153286

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T-1 3xFlag-p38alpha

   Plasmid

   #47575

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   p38alpha (Mapk14 Mouse)
   

   Use

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-N1-Cdc42BPB

    Plasmid

    #50759

    Purpose

    expression of EGFP tagged Cdc42BPB

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    Cdc42BPB (CDC42BPB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    6His, TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDARMO_CMVT_FLAG-HUWE1

    Plasmid

    #187121

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-trGluc

    Plasmid

    #158959

    Purpose

    DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    trGluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-C2-mKate

    Plasmid

    #208763

    Purpose

    To express C2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINS-USP21 (USP, aa 196-565)

    Plasmid

    #61585

    Purpose

    Expresses human USP21 (USP domain) in E. coli.

    Depositor

    David Komander

    Article

    Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

    Insert

    USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Deleted aa 1-195.

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEXtev-HMG54kd

    Plasmid

    #26030

    Depositor

    Johann Deisenhofer

    Article

    Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

    Insert

    3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Catalytic domain (amino acids 426-488) Methion…

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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