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pHB1

Plasmid

#204668

Purpose

pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

Depositor

Mark Mandel

Article

Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

Insert

FRT-erm-FRT

Use

Synthetic Biology

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrAB-AsLOV2*(543)

Plasmid

#210863

Purpose

IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Inserts

acrA
acrB

Use

Synthetic Biology

Tags

AsLOV2*(543)

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-GFP

Plasmid

#205724

Purpose

Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-GFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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aCE89

Plasmid

#204494

Purpose

TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

φC31 integrase
WBeta recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

PhIF and TREtight

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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aCE90

Plasmid

#204495

Purpose

TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

phiC31 recombinase
WBeta recombinase

Use

Synthetic Biology

Tags

PEST

Expression

Bacterial and Mammalian

Promoter

PhIF and TREtight

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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nUnaG-ST

Plasmid

#207630

Purpose

A plasmid encoding the N-terminal UnaG fragment fused to SpyTag.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

nUnaG-SpyTag-MBP

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-84

Promoter

T7

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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TaraACR1-pmCherry-C1

Plasmid

#204960

Purpose

Expression of TaraACR1 in fusion with mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

TaraACR1

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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TaraACR2-pmCherry-C1

Plasmid

#204961

Purpose

Expression of TaraACR2 in fusion with mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

TaraACR2

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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TaraACR3-pmCherry-C1

Plasmid

#204962

Purpose

Expression of TaraACR3 in fusion with mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

TaraACR3

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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S16-ACR1-pcDNA3.1-mScarlet

Plasmid

#204963

Purpose

Expression of S16-ACR1 fused to mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

S16-ACR1

Tags

mScarlet

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pActin-miRFP704nano

Plasmid

#206191

Purpose

Human beta-actin labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano-human beta-actin

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP704ano

Plasmid

#206196

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

H2B-miRFP704nano

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLAMP1-miRFP704nano

Plasmid

#206195

Purpose

LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

LAMP1-miRFP704nano

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pATT-Dest-Cre

Plasmid

#202714

Purpose

Cre recombinase staging plasmid to make transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Cre

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTKEI-Dest-loxP-sfGFP

Plasmid

#202715

Purpose

Empty expression vector containing Cre recombinase reporter.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Pw7-loxP-term-loxP-sfGFP

Use

Cre/Lox

Expression

Bacterial

Promoter

constitutive

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_18 [AMA1 long ORI]

Plasmid

#198931

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

AMA1 long ORI

Use

Synthetic Biology

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF_TypeIC_target_sfGFP_invivo

Plasmid

#196399

Purpose

expresses CRISPR Cascade Type IC target sequence for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

type IC dsDNA target

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIC4_invivo

Plasmid

#196403

Purpose

expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IC4 protein

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_15 [CEN6/ARSH4 ORI]

Plasmid

#198928

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

CEN6/ARSH4 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_175

Plasmid

#199107

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Dummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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