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pUC19_CRISPR_DpmrA

Plasmid

#160903

Purpose

CRISPR_Cas9, lambda red recombineering

Depositor

Anne-Catrin Uhlemann

Article

McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

Inserts

Cas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette

Promoter

araBAD

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shTREX1

Plasmid

#127700

Purpose

Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shTREX1 (Trex1 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAM233

Plasmid

#226655

Purpose

pcDNA3.1-p97-FLAG

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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GST-beta-arrestin1

Plasmid

#36918

Depositor

Robert Lefkowitz

Article

Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

GST

Expression

Bacterial

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only
MT3

Plasmid

#105710

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 3

Depositor

Artur Krężel

Article

Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

Insert

Metallothionein 3 (MT3 Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-hGSDMC

Plasmid

#218937

Purpose

Expression of N-terminal FLAG tagged human gasdermin C

Depositor

Christopher LaRock

Article

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

human GSDMC (GSDMC Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-hGSDMB

Plasmid

#218936

Purpose

Expression of N-terminal FLAG tagged human gasdermin B

Depositor

Christopher LaRock

Article

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

human GSDMB (GSDMB Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG2IG-Hmga1a

Plasmid

#122293

Purpose

Overexpression for mouse Hmga1a

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Hmga1a (Hmga1 Mouse)

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin

Plasmid

#23955

Purpose

Mammalian expression of human Park2 fused to EYFP

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Park2 (PRKN Human)

Tags

eYFP

Expression

Mammalian

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only

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pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
Agrp-pGL3

Plasmid

#17552

Depositor

Domenico Accili

Article

Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Insert

Agrp (Agrp Mouse)

Use

Luciferase

Mutation

Contains ~400bp of the mouse Pomc gene promoter, …

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDY1656 STITCHR Gluc AAVS1 payload

Plasmid

#234827

Purpose

Gluc exon 2 with 50bp AAVS1 homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

Gluc exon 2

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS928

Plasmid

#238211

Purpose

Expresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

HA-NLS

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ADAM17-HA

Plasmid

#65105

Purpose

Expression of HA tagged ADAM17 (TACE) in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-anti-AlfaTag nanobody

Plasmid

#171818

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

cfSGFP2-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ADAM10-HA

Plasmid

#65106

Purpose

Expression of HA tagged ADAM10 in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDY1513 AAVS1 sgRNA

Plasmid

#234832

Purpose

sgRNA for AAVS1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

AAVS1 sgRNA

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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