We narrowed to 11,713 results for: NSI

1. pHH0103_BCAR1-1/1

   Plasmid

   #91418

   Purpose

   Protein expression and purification of human SH3 domain construct BCAR1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   BCAR1-1/1 (BCAR1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX VAMP7 (1-188)

   Plasmid

   #42315

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Δ181-220

   Promoter

   Ptac

   Available Since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pHH0103_FYN-1/1

   Plasmid

   #91413

   Purpose

   Protein expression and purification of human SH3 domain construct FYN-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   FYN-1/1 (FYN Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC_hlbCas12a-Nlux

   Plasmid

   #176240

   Purpose

   pNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoter

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized lbCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Promoter

   Ribosomal subunit

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHES795

   Plasmid

   #87943

   Purpose

   Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   Zif268 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
   

   Expression

   Yeast

   Promoter

   ADH1 (crippled)

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC_hfnCas12a_NR1_NR2_LPT1

   Plasmid

   #176253

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT gene

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF-DEST51-GOLPH3

   Plasmid

   #21690

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Invitrogen gateway destination vector

   Tags

   6xHIS and V5

   Expression

   Mammalian

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. PXPR007 sgCIC-1

   Plasmid

   #74953

   Purpose

   Cas9 + sgCIC-1 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   CIC (CIC Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. K-to-R mutant NPRL2(1-380) in pLJM60

   Plasmid

   #184562

   Purpose

   CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

   Depositor

   Kacper Rogala

   Article

   Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

   Insert

   NPRL2 (NPRL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   All lysines were mutated to arginines.

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBacI-KDAC6

    Plasmid

    #224346

    Purpose

    Expresses human KDAC6 (HDAC6) in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC6 (HDAC6 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. FUW-TetO-Tead4

    Plasmid

    #128832

    Purpose

    dox-dependent expression of mouse Tead4

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Tead4 (Tead4 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XFL-Src-S4-S32-KD

    Plasmid

    #140299

    Purpose

    Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST40-2XFL-Src-S32-KD

    Plasmid

    #140311

    Purpose

    Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the SH3, SH2, and KD regions

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiRCas9-Lambda2

    Plasmid

    #104184

    Purpose

    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

    Depositor

    Eugene Yeo

    Article

    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

    Insert

    U6-sgRNA, EFS-PIN-dCas9
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HR-TERT-SV40-GFP

    Plasmid

    #71397

    Purpose

    Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Tags

    SV-40 GFP insertion in TERT promotor

    Promoter

    Endogenous TERT promotor

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAC_N_LMNA

    Plasmid

    #111688

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    LMNA (LMNA Human)
    

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-EGFP-CAAX

    Plasmid

    #208684

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AP1muA single guide RNA

    Plasmid

    #230030

    Purpose

    guide RNA for AP1muA tagging at the C-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    AP1M1 guide (AP1M1 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMNZ-neo-Flag-TNFR1 L380A

    Plasmid

    #43949

    Depositor

    Martin Kluger

    Article

    D'Alessio et al J Biol Chem. 2010 Jul 30;285(31):23868-7

    Insert

    Tumor Necrosis Factor Receptor 1 (TNFRSF1A Human)
    

    Tags

    Flag*

    Mutation

    L380A

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCW-Cdt1ΔCy-mCherry-Puro

    Plasmid

    #193764

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1ΔCy-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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