We narrowed to 851 results for: gfp cassette

1. pLV-Hygro-EF1A-BRCT2-Linker-eGFP

   Plasmid

   #176085

   Purpose

   EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT2 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Hygro-EF1A-BRCT1-Linker-eGFP

   Plasmid

   #176074

   Purpose

   EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-PGK-scFv GCN4-sfGFP-VP64

   Plasmid

   #122133

   Purpose

   Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   scFv GCN4-sfGFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-CMV-EGFP-PolB-PAMmut-Hygro

   Plasmid

   #176086

   Purpose

   EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in the PAM site used by PolBKOg1

   Promoter

   CMV

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5` CC: Puro – loxP – GFP-C

   Plasmid

   #219563

   Purpose

   5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   hPGK-promoter_PuroR_2A_loxP_GFP-C
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HR_G3BP1-V5-APEX2-GFP

   Plasmid

   #105284

   Purpose

   homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
   

   Use

   Human homologous recombination donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-PARP1-del.p119K120S–eGFP

   Plasmid

   #211554

   Purpose

   A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of amino acids 119K 120S

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

   Plasmid

   #92140

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

   Plasmid

   #86230

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82705

    Purpose

    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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