We narrowed to 1,134 results for: fret

1. Epac-S-H127

   Plasmid

   #170343

   Purpose

   6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Insert

   6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-Pur-Lyn-AktAR2-EV-D

   Plasmid

   #125200

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Insert

   Lyn-AktAR2-EV-D
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-AKAR4

   Plasmid

   #61619

   Purpose

   FRET-based biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4
   

   Tags

   Cerulean and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETA_crGE

   Plasmid

   #172927

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRF1-GW AKAR3-EV

   Plasmid

   #182533

   Purpose

   Expresses the PKA FRET sensor AKAR3-EV in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

   Insert

   AKAR3-EV
   

   Expression

   Yeast

   Promoter

   PMA1

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. vgCam-pRSETb

   Plasmid

   #193362

   Purpose

   A FRET-based calcium probe using Sumire

   Depositor

   Takeharu Nagai

   Article

   Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

   Insert

   vgCam
   

   Use

   E.coli expression

   Tags

   His X6 tag and Strep tag

   Promoter

   T7

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLIParaF.Ec-250n

   Plasmid

   #18855

   Depositor

   Wolf Frommer

   Article

   Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

   Insert

   arabinose FRET sensor
   

   Tags

   CFP and Venus

   Expression

   Bacterial

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pFLIPmal-40uDelta1YFP

   Plasmid

   #18853

   Depositor

   Wolf Frommer

   Article

   Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

   Insert

   optimized maltose FRET sensor
   

   Tags

   CFP and YFP

   Expression

   Bacterial

   Mutation

   N227A;

   Available Since

   Oct. 23, 2008

   Availability

   Academic Institutions and Nonprofits only

10. 234_pSKB3-Venus-LUMP

    Plasmid

    #65975

    Purpose

    Bacterial expression of Venus-LUMP FRET pair

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Insert

    LUMP
    

    Tags

    Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. cp173-mCer3-C1

    Plasmid

    #105752

    Purpose

    a circularly permuted mCerulean3 for FRET sensor optimization

    Depositor

    Megan Rizzo

    Article

    Markwardt et al J Biol Chem. 2016 Feb 5;291(6):3000-9. d

    Insert

    cp173-mCer3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Lyn-AKAR4

    Plasmid

    #61620

    Purpose

    Lipid raft-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    Lyn-AKAR4
    

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 GfaABC1D NAPA-A SV40

    Plasmid

    #92281

    Purpose

    FRET probe; extracellular membrane tethered eGFP

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Astrocyte
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-CaNAR2

    Plasmid

    #64728

    Purpose

    FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Tags

    Cerulean3 and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDN3.1(+)-CaNARi

    Plasmid

    #64848

    Purpose

    FRET-based calcineurin (CaN) activation biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSETA_crE6

    Plasmid

    #172928

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Camk2-NES-Twitch-GR

    Plasmid

    #173044

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-GR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-AktAR2

    Plasmid

    #64932

    Purpose

    Enhanced FRET-based biosensor for monitoring Akt activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    AktAR2
    

    Tags

    Cerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. piGECInano-N1

    Plasmid

    #186190

    Purpose

    Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

    Insert

    iGECInano
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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