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MBP-ATG13 (363-517)-ULK1 (836-1050)-His

Plasmid

#222824

Purpose

Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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His-GST-ATG13 (363-517)-ULK1 (836-1050)

Plasmid

#222822

Purpose

Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP

Plasmid

#222821

Purpose

Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

TwinStrep Flag and msfGFP-10His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-tagged Sox17FNV

Plasmid

#206391

Purpose

Expresses Halo-tagged mouse SOX17FNV in mammalian cells, for single-molecule tracking.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Sox17FNV (Sox17 Mouse)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mBrn2-3xflag

Plasmid

#206390

Purpose

Expresses 3xflag fused mouse BRN2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-FHIP

Plasmid

#198527

Purpose

Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

FHIP (FHIP1B Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

silent mutations in codons 353 and 651 as well as…

Promoter

ADH1

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H277Y-HA

Plasmid

#198185

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 277 mutates to …

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-FTS

Plasmid

#198526

Purpose

Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

FTS (AKTIP Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-FHIP-L

Plasmid

#198528

Purpose

Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

FHIP-L (FHIP1A Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook1

Plasmid

#198523

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook1 (HOOK1 Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

silent substitution in codon 620 to eliminate int…

Promoter

ADH1

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B H85E K88E I92E

Plasmid

#175033

Purpose

Expresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (H85E K88E I92E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Histadine 85 to Glutamate (CAC to GAG); Lysine 88…

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ150-x3.7

Plasmid

#124308

Purpose

Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9-x3.7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ151D10A-x3.7

Plasmid

#124309

Purpose

Gateway entry vector with pcoCas9D10A-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-x3.7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

Plasmid

#121509

Purpose

Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah.intron9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgIdua.CMV/CB-EGFP

Plasmid

#121511

Purpose

Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgIdua

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgAspa.CMV/CB-EGFP

Plasmid

#121510

Purpose

Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgAspa

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3_FAM153CP

Plasmid

#232377

Purpose

Enhancer near FAM153CP

Depositor

Joanna Wysocka

Insert

FAM153CP (FAM153CP Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pZNF608-donor

Plasmid

#176362

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

ZNF608 (ZNF608 Human)

Use

CRISPR

Tags

GFP

Expression

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-TEV-SRC(WT)

Plasmid

#223742

Purpose

Expression of recombinant protein for purification, codon optimized for Insect cells

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

Src Kinase (SRC Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Ezh2[FL]-dCas9

Plasmid

#100086

Purpose

Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

14056 (Ezh2 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Ezh2[FL]-dCas9-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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