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KG#707

Plasmid

#110911

Purpose

Expresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neurons

Depositor

Kenneth Miller

Article

Harterink et al J Cell Sci. 2018 Oct 22;131(20). pii: jc

Insert

sup-1 minigene

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeALG1_O

Plasmid

#147147

Purpose

Insect Expression of CeALG1

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeALG1

Expression

Insect

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159860

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RFP-RGG

Plasmid

#124944

Purpose

Exoression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His and mCherry

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJH2069

Plasmid

#179224

Purpose

Pnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-9 (unc-9 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKS2311

Plasmid

#37835

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1W32A;Y35A::YFP (cpl-1 Nematode)

Tags

YFP

Expression

Worm

Mutation

W32A;Y35A

Promoter

nhx-2

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pMLS719

Plasmid

#188894

Purpose

miniMos Arm sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(K09B11.12)::miniMos Arm sgRNA

Expression

Worm

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS EFF-1::V5

Plasmid

#133584

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

Insert

EFF-1::V5

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1273

Plasmid

#44487

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCM1.109

Plasmid

#19597

Depositor

Geraldine Seydoux

Insert

pgl-3 prom in PDONRP4P1R

Expression

Worm

Available Since

Jan. 2, 2009

Availability

Academic Institutions and Nonprofits only

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TC393

Plasmid

#122625

Purpose

Pser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Expression

Worm

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Plasmid

#159801

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPK719

Plasmid

#38149

Depositor

Patricia Kuwabara

Insert

cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)

Use

Recombineering

Expression

Worm

Promoter

unc-119

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pGFPmAID_htz-1

Plasmid

#174214

Purpose

Donor plasmid for tagging htz-1 with GFP::mAID

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

GFP::mAID::htz-1 tagging donor

Use

CRISPR

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJH1922

Plasmid

#132361

Purpose

Prgef-1 INS-1::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-1::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-1 (ins-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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