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Plasmid#220066PurposesiRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophoreDepositorInsertOSBPL5 siRNA resistant (OSBPL5 Human)
UseTagsExpressionMammalianMutationsiRNA resistantPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLBC0120
Plasmid#172791PurposeLevel 0 module containing CDS2ns_mHSP21DepositorInsertCDS mature HSP21 (44-227), Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0130
Plasmid#172792PurposeLevel 0 module containing CDS2ns_mPTAC5DepositorInsertCDS mature PTAC5 (41-387), Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:: pRPS5a-PE2max-NC::tHSP
Plasmid#227694PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.DepositorInsertPE2max-NC being driven by pRPS5a and terminated by tHSP
UseCRISPRTagsExpressionPlantMutationPromoterpRPS5aAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
(+)-Limonene Synthase
Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
UseTagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52PromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
35S::GeNL/pCAMBIA1301
Plasmid#190070PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thalianaDepositorInsertGreen-enhanced Nano-lantern
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AL12R-HSP101
Plasmid#161014PurposePP7-tagged reporter driven by the Arabidopsis HSP101 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCP-tRNA
Plasmid#133812PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisDepositorInsertcassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC1640
Plasmid#158719PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC2085
Plasmid#158721PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pY2F
Plasmid#53285PurposeContains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETCON-PTX-S1-220
Plasmid#67747PurposePertussis Toxin Subunit 1 Yeast Display PlasmidDepositorInsertPTxS1-220
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AL12R-HSP70
Plasmid#161010PurposePP7-tagged reporter driven by the Arabidopsis HSP70 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
UseTagsExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb-GAPC2
Plasmid#161009PurposePP7-tagged reporter driven by the Arabidopsis GAPC2 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtipiTrc
Plasmid#53275PurposeContains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.DepositorInsertipi1 (IPP2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Mtb-TF
Plasmid#111513PurposeExpresses M. tuberculosis Trigger Factor with a C-terminal His tagDepositorInsertTrigger Factor
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315-PGK-SEIPIN1
Plasmid#96986PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTagsExpressionYeastMutationPromoterPhosphoglycerate Kinase (PGK) promoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLBC0010
Plasmid#172774PurposeLevel 0 module containing NT1_CTPSSUDepositorInsertGene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only