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GFP-DroshaK382R

Plasmid

#62519

Purpose

Lysine 382 of Drosha was mutated to arginine

Depositor

Bharat Ramratnam

Article

Tang et al PLoS One. 2013 Aug 29;8(8):e72503. doi:

Insert

human Drosha with lysine 382 changed to arginine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed lysine 383 to arginine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
ΔCT163-GFP

Plasmid

#33116

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 163 aa of the variable cytop…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
ΔCT183-GFP

Plasmid

#33117

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 183 aa of the variable cytop…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
GFP-Rab19

Plasmid

#31741

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab19 (Rab19 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
GFP-Rab40

Plasmid

#31745

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab40 (Rab40 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179010

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179009

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK30 pHomL-RPL18Bp-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179014

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK29 pHomL-TEF2p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179013

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179011

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

Plasmid

#169077

Purpose

Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG7 (ATG7 Human)
ATG10 (ATG10 Human)
ATG12 (ATG12 Human)
ATG16 (ATG16L1 Human)

Use

Tags

GFP tag, GGlinker, TEV cleavage site. StrepII and…

Expression

Bacterial and Insect

Mutation

Promoter

Polyhedrin promoter

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

Plasmid

#48287

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and msfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

Plasmid

#48297

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

Plasmid

#55221

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

Plasmid

#225442

Purpose

BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

Depositor

Diego Orzaez

Insert

LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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p2.0VPI.EGFP

Plasmid

#40868

Depositor

Harold Gainer

Article

Ponzio et al PLoS One. 2012;7(11):e48860. doi: 10.137

Insert

mouse vasopressin promoter

Use

AAV

Tags

Expression

Bacterial and Insect

Mutation

Promoter

mouse vasopressin

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBacMam3_N_His8_eGFP_3C

Plasmid

#178807

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

His8, eGFP, 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacMam4_N_Flag_His8_eGFP_TEV

Plasmid

#178808

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

Flag, His8, eGFP, TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge