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pKDsgRNA-lpoB

Plasmid

#89959

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

lpoB gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_3

Plasmid

#73527

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_4

Plasmid

#73528

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_2

Plasmid

#73526

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pHU6_gRNA_Ch10-for1

Plasmid

#81210

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-rev1

Plasmid

#81208

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA_zebrafish_mmp21

Plasmid

#72890

Purpose

plasmid to generate guideRNA against mmp21 gene in zebrafish

Depositor

Nicholas Katsanis

Article

Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

Insert

zebrafish mmp21 (mmp21 Zebrafish)

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7 RNA pol

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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LsgRNA-MS2

Plasmid

#235597

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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HaloXRCC4 sgRNA

Plasmid

#207606

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of XRCC4.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

TACTGGGTTCAGAAACAAGG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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HaloKu70 sgRNA

Plasmid

#207583

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

GAGCAGTAGCCAACATGTCA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJC.15A.sgRNA.TA

Plasmid

#232483

Purpose

sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Inserts

Toxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

P3 constitutive promoter and native promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_B2M_sgRNA(PP7)

Plasmid

#232438

Purpose

sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_nsgRNA(PP7)

Plasmid

#232434

Purpose

nsgRNA for PE3b correction of the rd6 mutation, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

PP7-tagged nicking gRNA (PE3b) for rd6 correction driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-sgRNA006_CTCF

Plasmid

#232928

Purpose

Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9 (Ctcf S. pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA047_Podxl

Plasmid

#232932

Purpose

Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Podxl S. pyogenes)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbATML1-1pro

Plasmid

#231151

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbATML1-1pro

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbSTM-5'UTR

Plasmid

#231150

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbSTM-5?UTR

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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