176,531 results

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pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Publication

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

Depositor

Michael Lin

Publication

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Promoter

human synapsin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lamp1_mStayGold

Plasmid

#221902

Purpose

Expresses mStayGold fused to Lamp1

Depositor

Janelia Integrative Imaging

Insert

Lamp1_mStayGold

Expression

Mammalian

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-CD19 puro

Plasmid

#196634

Purpose

Express CD19

Depositor

Nicolas Manel

Publication

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

CD19 (CD19 Human)

Use

Lentiviral

Mutation

WT

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi G418R mbIL21

Plasmid

#194329

Purpose

Consitiutive mammalian expression of membrane bound human IL-21

Depositor

Alexander McLellan

Publication

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

mbIL-21 (IL21 Human)

Expression

Mammalian

Promoter

EF1-a

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1_blasti

Plasmid

#194067

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDARMO_HUWE1_3xFLAG

Plasmid

#187155

Purpose

mammalian cell expression of HUWE1-3xFLAG

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP-KDEL

Plasmid

#184904

Purpose

Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

Depositor

Noboru Mizushima

Publication

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

KDEL

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBMCL1

Plasmid

#178203

Purpose

An improved vector for BacMam system

Depositor

Lei Chen

Publication

Guo et al Biochem Biophys Res Commun. 2022 Feb 26;

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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CoV2-N-R203M

Plasmid

#177952

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203M) (N )

Expression

Mammalian

Mutation

R203M

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-dCasRx-ALKBH5-PURO

Plasmid

#175582

Purpose

Expresses dCasRx-ALKBH5 in mammalian cell

Depositor

Qi Xie

Publication

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-ALKBH5

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RfxCas13d R239A/H244A/R858A/H863A

Promoter

pMSCV

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLentiCMV-Blast

Plasmid

#125133

Purpose

Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

Depositor

Jin Chen

Publication

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Type

Empty backbone

Use

Lentiviral

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Publication

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Publication

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Publication

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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C1-F-tractin-mCherry

Plasmid

#155218

Purpose

Monitor global F-actin distribution

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)

Tags

mCherry

Expression

Mammalian

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Publication

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPR-HOT_tdTomato

Plasmid

#138567

Purpose

Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

Depositor

Hans Clevers

Publication

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

tdTomato

Use

Unspecified

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Publication

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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