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TFORF3168

Plasmid

#144644

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Cavin4-mCherry

Plasmid

#176007

Purpose

Mammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4 (Cavin4 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1b

Plasmid

#176025

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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S10A mutant of H3S10ph biosensor

Plasmid

#120810

Purpose

FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP

Expression

Mammalian

Mutation

Serine 10 is mutated to Alanine, and Threonine 3,…

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LILRA5-Fc(DAPA)-AviTag-6xHis

Plasmid

#156677

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRA5 (LILRA5 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Cavin4a in pBluescript(SK+)

Plasmid

#126567

Purpose

For generating RNA probes for in situ hybridisation. Parton lab clone ISB

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Use

Gateway entry clone

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag NEK11

Plasmid

#20546

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NEK11 (NEK11 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
Anti-Kv2.1 K+ channel [D4/11R]

Plasmid

#188179

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (Rat). Derived from hybridoma D4/11

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 T73I

Plasmid

#140939

Purpose

expresses PTPN11 T73I

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Tags

V5

Expression

Mammalian

Mutation

T73I

Promoter

EF1a

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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W118-1_hRSK3(RPS6KA2)_K91A_K444A

Plasmid

#180385

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2_K91A/K444A (RPS6KA2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

K91A/K444A

Promoter

CMV

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel [L83/11R]

Plasmid

#177490

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (Rat). Derived from hybridoma L83/11.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 E76G

Plasmid

#140862

Purpose

expresses PTPN11 E76G

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Expression

Mammalian

Mutation

E76G

Promoter

EF1a

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC11A2

Plasmid

#251684

Purpose

gRNA to knock out SLC11A2 in mammalian cells

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

SLC11A2 solute carrier family 11 member 2 (SLC11A2 Human)

Use

CRISPR and Lentiviral

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTR01F/hFGFR3b-V5

Plasmid

#236016

Purpose

for PiggyBac mediated integration and stable expression of hFGFR3b protein

Depositor

Pavel Krejčí

Article

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

human FGFR3b (FGFR3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

truncated CMV promoter

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR2 [K67/11R]

Plasmid

#206606

Purpose

Mammalian Expression Plasmid of anti-CASPR2 (Human). Derived from hybridoma K67/11.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CASPR2 (Homo sapiens) recombinant Mouse monoclonal antibody (CNTNAP2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

Plasmid

#209199

Purpose

Mutagenesis of Kcnma1

Depositor

Larry Zweifel

Article

Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

Insert

Kcnma1 (Kcnma1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-FGF11 [N92/14R]

Plasmid

#206602

Purpose

Mammalian Expression Plasmid of anti-FGF11 (Human). Derived from hybridoma N92/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-FGF11 (Homo sapiens) recombinant Mouse monoclonal antibody (FGF11 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shAtxn1

Plasmid

#180387

Purpose

Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shAtxn1 (Atxn1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1b

Plasmid

#176021

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

EGFP

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-Cavin4a-mCherry

Plasmid

#176022

Purpose

Leishmania cell free expression of zebrafish Cavin4a-mCherry. Parton lab clone GWU

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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