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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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pg50-2.11 (#65)

Plasmid

#35966

Depositor

Thomas Sato

Publication

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Insert

Tie2 promoter-1st exon-1st intron

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-caAkt-P2A-AktKiMBImut

Plasmid

#256283

Purpose

Non-responsive mutant control for Akt KiMBI

Depositor

Michael Lin

Publication

Wu et al Nat Chem Biol. 2025 Aug;21(8):1194-1204.

Insert

caAkt-P2A-AktKiMBImut

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Aug. 24, 2026

Availability

Academic Institutions and Nonprofits only

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PAL-AA246-368-GFP

Plasmid

#221590

Purpose

Expresses the palmitolylation region of mamalian Arl13b (amino acids 246-368) and GFP-tagged

Depositor

Lei Lu

Publication

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acid 10-245,369-428

Promoter

CMV promoter

Available since

May 22, 2026

Availability

Academic Institutions and Nonprofits only

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AA245-428-GFP

Plasmid

#221174

Purpose

Expresses mammalian Arl13b with amino acids 245-428 and GFP-tagged

Depositor

Lei Lu

Publication

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-244

Promoter

CMV promoter

Available since

May 22, 2026

Availability

Academic Institutions and Nonprofits only

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pKG1117_pPV0-pShip_backbone

Plasmid

#239685

Purpose

Backbone for BsaI Golden Gate assembly, allows gene to be inserted into pHarbor backbones in subsequence PaqCI Golden Gate assembly

Depositor

Kate Galloway

Publication

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(1-376)-NC-LZ-mNG

Plasmid

#253325

Purpose

mammalian expression of the motor domain (aa 1-376) of human KIF1C tagged with mNG. The NC and LZ sequences facilitate stable dimerization of the motor domains.

Depositor

Kristen Verhey

Publication

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(1-376) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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PAL-AA246–324-GFP

Plasmid

#221591

Purpose

Expresses the palmitolylation region of mamalian Arl13b (amino acids 246-325) and GFP-tagged

Depositor

Lei Lu

Publication

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acid 10-246,325-428

Promoter

CMV promoter

Available since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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PAL-AA248-428-GFP

Plasmid

#221176

Purpose

Expresses the palmitolylation region and amino acids 248-428 of mamalian Arl13b and GFP-tagged

Depositor

Lei Lu

Publication

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acid 10-247

Promoter

CMV promoter

Available since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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AA1-245-GFP

Plasmid

#247082

Purpose

Expresses mammalian Arl13b with amino acids 1-245 and GFP-tagged

Depositor

Lei Lu

Publication

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 246-428

Promoter

CMV promoter

Available since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1583_ECOR31HamA(E138AK140A)B_CoPurification

Plasmid

#223372

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)

Depositor

Jennifer Doudna

Publication

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)

Tags

MBP-His-TEV

Expression

Bacterial

Mutation

HamA E138A, K140A

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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Flag-Src C185A H201A H492A E524A

Plasmid

#246427

Purpose

Expresses Src pH insensitive mutant in Mammalian cells

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed C188 to A, H204 to A, H495 to A, and E527…

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJL309

Plasmid

#243705

Purpose

A piggyBac vector encoding an NDUFB9-mNeonGreen2(11) fusion protein that lacks a mitochondrial targeting sequence.

Depositor

Jonathan Weissman

Publication

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

NDUFB9 (NDUFB9 Human)

Tags

mNeonGreen2(11)

Expression

Mammalian

Mutation

mitochondrial targeting sequence deleted

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A E252A

Plasmid

#244493

Purpose

Bacterial Expression of pH insensitive SHP2 mutant

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine and Glutamate 25…

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A

Plasmid

#244492

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 E252A

Plasmid

#244491

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Glutamate 252 to Alanine

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src C185A

Plasmid

#246423

Purpose

Expresses Src mutant C185A or C188A in Mammalian cells

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Cys 188 to Ala

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRO275

Plasmid

#233825

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-1XOaf1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-1XOaf1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO276

Plasmid

#233826

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN102

Plasmid

#233828

Purpose

MoClo-YTK; type4A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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