We narrowed to 19,497 results for: INO

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pSBbi-NLSmCherryNLS Clover-HDHB

Plasmid

#190223

Purpose

Fluorescent cell cycle reporter

Depositor

Laura Heiser

Article

Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

Inserts

Human DNA Helicase B (amino acids 994-1087) (HELB Human)
NLS-mCherry-NLS-P2A-Puromycin

Use

Transposon

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Amino Acids 994-1087

Promoter

EF1a and RPBSA

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

Plasmid

#173029

Purpose

For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

Depositor

Mohamed Fareh

Article

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

pspCas13b

Use

CRISPR and Lentiviral

Tags

3xFLAG, BFP, HIV NES, and T2A

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Cre targeting vector

Plasmid

#61570

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb exon 4 - 2A - Cre

Use

Cre/Lox and Mouse Targeting

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_deltaP29_D408_pBabePuro

Plasmid

#58422

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids P29 to D408

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV1A

Plasmid

#224437

Purpose

Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDLTTP insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pscALPSpuro-MmACE2 (Mouse)

Plasmid

#158087

Purpose

Expresses mouse ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Mouse ACE2 (Ace2 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4E

Plasmid

#224452

Purpose

Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDFNNT insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBFC0993

Plasmid

#231993

Purpose

CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

Depositor

Brady Cress

Article

Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

Inserts

dRfxCas13d
crRNA with negative control (RFP-targeting) spacer

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and pTet

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs T3950D

Plasmid

#83320

Purpose

DNA-PKcs T3950A

Depositor

Katheryn Meek

Article

Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

Activation loop phosphorylation site 3950 substit…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-GCaMP6F

Plasmid

#137123

Purpose

Intersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP MASTL G44S (siRNA resistant)

Plasmid

#191012

Purpose

Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Article

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Tags

EGFP

Expression

Mammalian

Mutation

G44S

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_314-HRI-V5

Plasmid

#202434

Purpose

Expression of HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-kz-CD20-Puro

Plasmid

#209759

Purpose

Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PB53x EF1-Dendra2-RPB1Amr

Plasmid

#81228

Purpose

expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

Depositor

Timothée Lionnet

Article

Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

Insert

Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)

Tags

Dendra2

Expression

Mammalian

Mutation

N792D alpha amanitin resistance mutation (Bartolo…

Promoter

EF1a

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AMBRA1-3xFLAG

Plasmid

#172605

Purpose

Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP MASTL E167D (siRNA resistant)

Plasmid

#191013

Purpose

Expresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Article

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Tags

EGFP

Expression

Mammalian

Mutation

E167D

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mGFP-hNET

Plasmid

#193364

Purpose

expression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

Depositor

Harald Sitte

Article

Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

Insert

human Norepinephrine Transporter (SLC6A2 Human)

Tags

monomeric GFP (mGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

Plasmid

#65458

Purpose

Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

tTA2

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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