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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,921 results for: pET

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Showing: 7601 - 7620 of 9921 results

  1. pfMDH_K295A

    Plasmid
    #67726
    Purpose
    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Publication
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 295 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. SULT1C2 (2AD1)

    Plasmid
    #25473
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SULT1C2 (SULT1C2 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. MAK3

    Plasmid
    #25499
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MAK3 (NAA50 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. EPHA2 (3KKA)

    Plasmid
    #25324
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    epha2.904.970 (EPHA2 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. KIF3B

    Plasmid
    #25254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF3B:S6-Q359 (KIF3B Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S6-Q359
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pEB018

    Plasmid
    #215568
    Purpose
    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus
    Depositor
    Robin Ohm
    Insert
    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    Use
    Fungal homologous recombination plasmid
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215568-plasmid-map-sequence-id-428736

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  14. DsRed +9e

    Plasmid
    #191766
    Purpose
    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e
    Depositor
    Daniel Gerlich
    Publication
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DsRed
    Tags
    N terminal fusion of positively charged polypetod…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191766-plasmid-map-sequence-id-383844

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  15. FnR8-10

    Plasmid
    #182569
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant
    Depositor
    Timothy Springer
    Publication
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182569-plasmid-map-sequence-id-357183

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  17. 6CKO (DOT1L)

    Plasmid
    #119962
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 1, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119962-plasmid-map-sequence-id-234358

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  19. phSOD1-E100G-N’His

    Plasmid
    #117706
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    E100G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117706-plasmid-map-sequence-id-227528

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  20. phSOD1-I113T-N’His

    Plasmid
    #117703
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    I113T
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    117703-plasmid-map-sequence-id-227513

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Showing: 7601 - 7620 of 9921 results