We narrowed to 9,521 results for: try

1. pET-Sac-Aβ(M1-42/C18C33)

   Plasmid

   #173759

   Purpose

   Expresses the AβC18C33 peptide.

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

   Insert

   Aβ(M1-42/C18C33) (APP Human)
   

   Expression

   Bacterial

   Mutation

   changed valine 18 to cysteine; changed glycine 33…

   Promoter

   T7

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCOTS-pyl-GFP(35TAG)

   Plasmid

   #92047

   Purpose

   This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   EGFP
   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Replicative expression plasmid for cyanobacteria …

   Tags

   Histag

   Mutation

   Tyrosine in position 35 of the GFP was mutated f…

   Promoter

   LeuP (native S. elongatus promoter), PpsbII, and …

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. BcLOV4 fusion tools screening plasmid #1

   Plasmid

   #174511

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR8-PHF19

   Plasmid

   #68864

   Purpose

   GW entry vector for PHF19

   Depositor

   Adrian Bracken

   Article

   Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

   Insert

   PHF19 (PHF19 Human)
   

   Use

   Gateway entry vector

   Promoter

   none

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. p5E-1.7kbfabp6

   Plasmid

   #159087

   Purpose

   p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Insert

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   

   Use

   Promoter fragment 5' entry vector for tol2 c…

   Promoter

   1.7kb fabp6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ230-RR

   Plasmid

   #108860

   Purpose

   LbCpf1 Gateway entry plasmid with G548R and K611R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G548R and K611R, Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-TEV-mClover3

   Plasmid

   #105795

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mClover3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-MCS-c-Fos-b-globin (JDW 1237)

   Plasmid

   #229829

   Purpose

   A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   c-fos minimal promoter and b-globin intron
   

   Use

   Gateway entry clone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR1A mKate2-hu_ncOGT WT

   Plasmid

   #154282

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   WT OGT

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104095

    Purpose

    Expresses TREAT-siRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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