We narrowed to 9,439 results for: try

1. pKK-FLAG-TEV

   Plasmid

   #105768

   Purpose

   Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ239-RR

   Plasmid

   #108862

   Purpose

   FnCpf1 Gateway entry plasmid with N623R and K687R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   N623R and K687R, Cpf1 is rice codon optimized

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

   Plasmid

   #174336

   Purpose

   The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   human Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   pHluorin inserted in the extracellular loop 2 of …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121816

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-TEV-mEGFP

   Plasmid

   #105794

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5 beta4 1355T

   Plasmid

   #16038

   Depositor

   Filippo Giancotti

   Article

   Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1355T: amino acids 1-1355

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

   Plasmid

   #133793

   Purpose

   U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   CjeCas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V2-TGAA linker sgRNA architecture from Kim et al.…

   Promoter

   U6

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-TAP(FLAG-HA) CYLD

   Plasmid

   #60031

   Purpose

   CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Retroviral

   Tags

   FLAG, HA, and IRES Puro

   Expression

   Mammalian

   Mutation

   Promoter

   LTR-driven expression

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pRSET-A2-77p-Venus

   Plasmid

   #64856

   Purpose

   Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

   Depositor

   Sriram Neelamegham

   Article

   Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

   Insert

   VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
   

   Use

   Tags

   7X His and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-3xNLS-OptoJNKi

    Plasmid

    #89740

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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