We narrowed to 16,177 results for: LEA
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Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-QES.i03.c01-TM
Plasmid#111845PurposeMammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 Env (BG505 SOSIP.664)
TagsCD5 leader sequence and TM helix from MHC class IExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF
Plasmid#201217PurposepCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTFDepositorAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-Mdm2 p60
Plasmid#52059Purposeexpression of truncated Mdm2 in mammalian cellsDepositorInsertMdm2 p60 (truncated) (MDM2 Human)
Tagsmyc3ExpressionMammalianMutationp60 (truncated at D367, DVDP motif)PromoterCMVAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-CA576-Z
Plasmid#89108Purposemammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576DepositorAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA
Plasmid#218914PurposePiggyBac transpositionDepositorInsertV5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
ExpressionMammalianMutation2363G>C and 4330T>CAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EphrinB3-Flag(A227Y)
Plasmid#155013PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1DepositorInsertEphrinB3 (EPHB3 Human)
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationA227YPromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2-Puro
Plasmid#169224PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2-P2A-Puro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Gata3
Plasmid#128824Purposedox-dependent expression of mouse Gata3DepositorInsertGata3 (Gata3 Mouse)
UseLentiviralAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-FKO (NC-flag)
Plasmid#159363PurposeMammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.DepositorInsertSARS-CoV-2 S protein (S SARS-CoV-2)
TagsFlag and FlagExpressionMammalianMutationFurin-cleavage site mutationPromoterCMVAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer
Plasmid#176589PurposeEndogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Chesapeake Bay) locus
ExpressionBacterialMutationTruncated CRISPR array to two direct repeats, rep…Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRFmut
Plasmid#208663PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVPromoterEFSAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Eomes
Plasmid#128825Purposedox-dependent expression of mouse EomesDepositorAvailable SinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NCBD + ACTR
Plasmid#99335PurposeBacterial coexpression of CBP NCBD and NCoA3 binding domainsDepositorExpressionBacterialPromoterT7Available SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL
Plasmid#119952PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
ExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…Available SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBactin-EGFP-NEDD1-gTBD
Plasmid#196857PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertEGFP-N-gTBD (NEDD1 Human)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-actin-pcDNA4-TO
Plasmid#118370PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.DepositorAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mOrange2-NEDD1-gTBD
Plasmid#196858PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD (NEDD1 Human)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA NC
Plasmid#176259PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI andDepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseMutationH840A and D10A mutations on spCas9 to inactivate …Available SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-HsSyx-375-792
Plasmid#179292PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + GST + TEV protease cleavage siteExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only