We narrowed to 15,548 results for: LEA

1. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

   Plasmid

   #112212

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   N-terminus of dCas9 and DNMT3A (DNMT3A Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H840A,N863A

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-dCTNNA1

   Plasmid

   #176032

   Purpose

   A knock-out vector for dog CTNNA1 (alpha-1-catenin)

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pelB-MBP-HtrA-ss

   Plasmid

   #137066

   Purpose

   E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature HtrA: lacks the HtrA signal peptide

   Promoter

   T7lac

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSEss-CHIg-mM-Asn209Ala_M18

   Plasmid

   #91733

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

   Insert

   immunoglobulin heavy constant mu (Ighm Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Asn209 changed to Ala (EU numbering)

   Promoter

   hEF1-HTLV prom

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-hAR-R617M,K618M,K632M,K633M

   Plasmid

   #115778

   Purpose

   Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

   Insert

   Androgen Receptor (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R617M, K618M ,K632M, K633M, insertion of Q81 and …

   Promoter

   CMV

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-Flag-SPRTN-Y117C

   Plasmid

   #110216

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

   Depositor

   Kristijan Ramadan, Ivana Marinovic-Terzic

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y117C, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-NLS-YFP-CCTd

   Plasmid

   #184325

   Purpose

   Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTd (CACNA1D Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/TO-myc-BirA*-cCE

   Plasmid

   #112713

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   BirA* (BirA R118G), HIV Rev nuclear localization …

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

   Plasmid

   #110761

   Purpose

   Bacterial expression of proISG15

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75244

    Purpose

    CRISPR/Cas9 plasmid against human Helios

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Helios (IKZF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid

    #85821

    Purpose

    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    Zf Ubi::cMyc oDam_f_GFPoNLS
    

    Use

    Tol2 based vector

    Tags

    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

    Expression

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pitx2_R (OZ534)

    Plasmid

    #27211

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting pitx2 (pitx2 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pitx2_L (OZ533)

    Plasmid

    #27210

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting pitx2 (pitx2 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pLL3.7-EF-EYFP-YAP1_Y357F-PolyA

    Plasmid

    #112287

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Tyrosine 357 to Phenyalanine corresponding to tyr…

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. yap1_R (OZ590)

    Plasmid

    #35230

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting yap1 (yap1 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. yap1_L (OZ589)

    Plasmid

    #35229

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting yap1 (yap1 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pMSCV40-EF1a-hBCL10(1-185)-SO

    Plasmid

    #75258

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Mutation

    AS 1-185 (w/o C-terminus)

    Promoter

    pMSCV-LTRs, EF1a

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. histamine receptor H3 (hrh3)_R (OZ550)

    Plasmid

    #28075

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting histamine receptor H3 (hrh3) (hrh3 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCDF-casBCDE(+6)

    Plasmid

    #89728

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

    Depositor

    Konstantin Severinov, Konstantin Kuznedelov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a longer versi…

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75238

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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