We narrowed to 10,265 results for: iCre

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dora[B]_rescue_construct

Plasmid

#190637

Purpose

Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMM4_14L

Plasmid

#183063

Purpose

Plasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Inserts

mCherry
sfpHluorin
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

RET2, Synthetic promoter, and TDH3

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pIPKAH

Plasmid

#185869

Purpose

Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pmRE-Tn5-142

Plasmid

#118531

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FluoSTEP-AKAR (C/Y)

Plasmid

#181862

Purpose

FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (C-Y)

Tags

CFP(1-10) and cpVenus(E127)

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pWP001

Plasmid

#178596

Purpose

pAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiT

Depositor

Robert Fagan

Article

Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

Insert

CBD-SmBiT/LgBiT

Expression

Bacterial

Promoter

Pxyl

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD2

Plasmid

#180602

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD1

Plasmid

#180601

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRBP-L2+D2+L3-myc/His

Plasmid

#180600

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-MGW-Cterm_H

Plasmid

#146484

Purpose

Insect Expression of DmGW182-MGW-Cterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182-MGW-Cterm (gw Fly)

Expression

Insect

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3_A

Plasmid

#145895

Purpose

Insect Expression of DmNot3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot3 (Not3 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_L139*

Plasmid

#174400

Purpose

S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

L139* (Truncation after 139th amino acid in sarA)

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_S130A

Plasmid

#174399

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

S130A

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_Y167F

Plasmid

#174398

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Y167F

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585-gp130

Plasmid

#174393

Purpose

Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Tags

Flag

Expression

Mammalian

Mutation

140-177 of sarA replaced with homologous IL6ST se…

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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