We narrowed to 15,732 results for: LEA

1. pcDNA3.1-IDO1-p2a-eGFP

   Plasmid

   #187026

   Purpose

   Expresses IDO1 and GFP (seperately) in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

   Insert

   Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
   

   Tags

   P2A-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-MyoAAV3A

   Plasmid

   #224442

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDYVGL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CRISPRv2-sgIl1r1_#2-hygro

   Plasmid

   #231990

   Purpose

   Knockout mouse Il1r1

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

   Plasmid

   #176279

   Purpose

   Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   GYG to AAA (aa144-146)

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATX3-13Q

   Plasmid

   #184248

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   C-terminal codon optimization for protein expres…

   Promoter

   T7 promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLHCX-ATF4 mScarlet NLS

   Plasmid

   #115970

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Human, Synthetic)
   

   Use

   Retroviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

   Plasmid

   #182652

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   4xU6-tRNAM15
   

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-FLAG-hHelios-IRES-GFP

   Plasmid

   #74047

   Purpose

   retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human Helios (IKZF2) (IKZF2 )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   encodes shorter version of human Helios

   Promoter

   pMSCV-LTRs

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

   Plasmid

   #187575

   Purpose

   Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SRSF1 (SRSF1 Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-BACE1-mBFP

    Plasmid

    #196697

    Purpose

    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Beta-secretase 1 (BACE1 Human)
    

    Tags

    HA and mBFP

    Expression

    Mammalian

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-DmrB-Casp8-2A-mCherry-puro

    Plasmid

    #231971

    Purpose

    Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Casp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro

    Plasmid

    #132773

    Purpose

    Expression of human LMNA.

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET OCaMP

    Plasmid

    #224932

    Purpose

    Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)

    Depositor

    Alexander Lohman

    Article

    Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

    Insert

    OCaMP
    

    Tags

    6xHis, RSET peptide, and Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAG-MCP-SRSF1-V5-mCherry

    Plasmid

    #235088

    Purpose

    SRSF1 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF1-V5-mCherry (SRSF1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-Bcr R1090A

    Plasmid

    #36416

    Depositor

    Eunjoon Kim, Nora Heisterkamp

    Article

    Oh et al J Neurosci. 2010 Oct 20;30(42):14134-44.

    Insert

    breakpoint cluster region protein isoform 1 (BCR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R1090A

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. kiCAP-AAV-MyoAAV1E

    Plasmid

    #224439

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDTMSK insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDule2-3-nitroTyrosine (A7)

    Plasmid

    #174079

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid

    #223137

    Purpose

    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-multi-CRISPR-sgCasp8_#2-puro

    Plasmid

    #231978

    Purpose

    Knockout mouse Casp8

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-multi-CRISPR-sgCasp8_#1-puro

    Plasmid

    #231977

    Purpose

    Knockout mouse Casp8

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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