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We narrowed to 16,337 results for: LEA

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Showing: 7641 - 7660 of 16337 results

  1. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

    Plasmid
    #208961
    Purpose
    Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208961-plasmid-map-sequence-id-415791

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  3. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

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  10. pelB-MBP-BB0323-ss

    Plasmid
    #137067
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0323: lacks the BB0323 signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137067-plasmid-map-sequence-id-291957

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  13. pLCHKO_intergenic-intergenic_2

    Plasmid
    #155078
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155078-plasmid-map-sequence-id-308534

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  15. pAAV-shMcu-2

    Plasmid
    #181867
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181867-plasmid-map-sequence-id-356033

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  16. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

    Plasmid
    #208976
    Purpose
    Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    nSpCas9(H840)-BPNLS-P2A-GFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    208976-plasmid-map-sequence-id-415837

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  20. CRHR2-DuET

    Plasmid
    #213216
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR2 (CRHR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213216-plasmid-map-sequence-id-430778

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Showing: 7641 - 7660 of 16337 results