We narrowed to 8,648 results for: FIE

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pCDFDuet-AtUBC8-S-AtUBA1-S

Plasmid

#222714

Purpose

Expression AtUBA1-S and AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtUBC8 (UBC8 Mustard Weed)
ATUBA1 (UBA1 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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N-BLa 1.2

Plasmid

#88999

Purpose

BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Promoter

araBAD

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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C-BLa 1.2

Plasmid

#89000

Purpose

BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

C-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Promoter

araBAD

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_SIE1

Plasmid

#136356

Purpose

Selection by Inducible Effectors Test Plasmid

Depositor

Andrew Goodman

Article

Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

Inserts

Type VI effector bfe1
Type VI effector bte1
BT_4676
tetR

Use

Unspecified

Mutation

ATG removed to add signal sequence before gene

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAV r3.45

Plasmid

#233684

Purpose

An evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cells

Depositor

David Schaffer

Article

Jang et al Mol Ther. 2011 Apr;19(4):667-75. doi: 10

Insert

Modified AAV2 Rep, Cap

Use

AAV

Mutation

insertion of LATQVGQKTA (where LA and A are linke…

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_HOIP/RNF31_#6

Plasmid

#75252

Purpose

retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against human RNF31 (RNF31 )

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-WT (AF41)

Plasmid

#21367

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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POLArISact

Plasmid

#164971

Purpose

Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0750

Plasmid

#185627

Purpose

Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

lacZ

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-LAP tag

Plasmid

#67618

Purpose

A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-termnal LAP (eGFP-TEV-S)

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pTone-gata2aECE-Kaede

Plasmid

#132976

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

Kaede
gata2a endothelial enhancer (x6) and a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7REwt

Plasmid

#213935

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Expression

Insect

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX50-HA

Plasmid

#165465

Purpose

Human DDX50 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DExD-box helicase 50 (DDX50 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA2

Plasmid

#164933

Purpose

Expresses EGFP sgRNA2 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/hTAZ-FL

Plasmid

#139979

Purpose

Expresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Full length human Tafazzin (TAFAZZIN Human)

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B

Plasmid

#136012

Purpose

EIF3B 3'UTR (NM_001037283.1) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.RGS4.C-HA

Plasmid

#154847

Purpose

Expresses murine RGS4 protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Regulator of G protein Signaling 4 (Rgs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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