We narrowed to 80,471 results for: MYC

1. pCMVtrunc msfGFPΔC9-LifeactΔN2

   Plasmid

   #231556

   Purpose

   Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

   Plasmid

   #175549

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   WAPL (Wapl Mouse)
   

   Use

   Mouse Targeting

   Tags

   AID[71-114]-eGFP

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB1677 REPAIR.t3

   Plasmid

   #176320

   Purpose

   CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Inserts

   human codon optimized Cas13bt3 (catalytically inactivated)
   huADAR2dd(E488Q) (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   HIV NES

   Expression

   Mammalian

   Mutation

   E488Q, only the deaminase domain (aa 276-702 are …

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_EF1a_Flag_TP53_R248W+P278A

   Plasmid

   #183115

   Purpose

   Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed proline 278 to alanine and arginine 248 t…

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIPZ-CTID195-GSQ-UBnc-PURO

   Plasmid

   #208037

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   Ubnc (UBC Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Mutation

   L73P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZE21/UBP1/ClpS_V65I

   Plasmid

   #98567

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   Mutated ATP-dependent Clp protease adapter protein
   

   Expression

   Bacterial

   Mutation

   Contains the V65I mutation that increases discrim…

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCVpuro-Mito-DsRed1

   Plasmid

   #87379

   Purpose

   Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

   Depositor

   Björn Stork

   Article

   Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

   Insert

   TXN2 mitochondrial transit peptide (TXN2 Human)
   

   Use

   Retroviral

   Tags

   DsRed1

   Expression

   Mammalian

   Mutation

   First 60 aa only

   Promoter

   Retroviral 5' LTR promoter

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDL52 (clone45)

   Plasmid

   #188578

   Purpose

   High-yield production of coenzyme F420 in E. coli

   Depositor

   Gerald Lackner

   Article

   Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

   Inserts

   ribA
   ribD
   yigB
   fbiC
   cofC
   cofD
   cofI
   cofE
   

   Expression

   Bacterial

   Mutation

   Codon optimization, synthetic ribosome binding si…

   Promoter

   T7 variants

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL-CRISPR.EFS.PAC

   Plasmid

   #57828

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. ptdTHC_PGKneoLox2DTA.2

    Plasmid

    #112625

    Purpose

    H2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    tdTomato

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-tdTHC

    Plasmid

    #112621

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-VHC

    Plasmid

    #112618

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-mTHC

    Plasmid

    #112620

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Tags

    mTeal (mTFP1)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVHC_PGKneoLox2DTA.2

    Plasmid

    #112622

    Purpose

    H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Venus

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGC-I2Cas9

    Plasmid

    #173158

    Purpose

    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Inserts

    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Plant

    Mutation

    Contains a D169N mutation with no visible effect …

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ptdTHC

    Plasmid

    #112617

    Purpose

    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Promoterless vector, ready for promoter for mamma…

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    No Promoter

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCB268

    Plasmid

    #53363

    Purpose

    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

    Depositor

    Alexander Varshavsky

    Article

    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

    Inserts

    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    

    Tags

    13xMyc, Flag, and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pVHC

    Plasmid

    #112614

    Purpose

    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Promoterless vector, ready for promoter for mamma…

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    No Promoter

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-EHC

    Plasmid

    #112619

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEHC

    Plasmid

    #112615

    Purpose

    promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Promoterless vector, ready for promoter for mamma…

    Tags

    Emerald

    Expression

    Mammalian

    Promoter

    No Promoter

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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