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pDMJ2-SARS-CoV-2-Spike (B.1-9c)

Plasmid

#241053

Purpose

Expresses SARS-CoV-2 spike (B.1-9c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,R158A,T478K,E484P,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9a)

Plasmid

#241051

Purpose

Expresses SARS-CoV-2 spike (B.1-9a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9b)

Plasmid

#241052

Purpose

Expresses SARS-CoV-2 spike (B.1-9b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,T478K,E484V,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8c)

Plasmid

#241049

Purpose

Expresses SARS-CoV-2 spike (B.1-8c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8d)

Plasmid

#241050

Purpose

Expresses SARS-CoV-2 spike (B.1-8d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8a)

Plasmid

#241047

Purpose

Expresses SARS-CoV-2 spike (B.1-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,R158A,K147N,N439R,T478K,S494R,F490R,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8b)

Plasmid

#241048

Purpose

Expresses SARS-CoV-2 spike (B.1-8b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,R403T,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-5a)

Plasmid

#241046

Purpose

Expresses SARS-CoV-2 spike (B.1-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,N481T,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-NR5A1-deltaLBD-FLAG

Plasmid

#242228

Purpose

Expression of human SF-1 ∆LBD (deletion of a.a. 221-459) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

NR5A1 (NR5A1 Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted ligand binding domain (LBD) from SF-1 (de…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV mTagBFP2-2A-CHMP2B-Q165X

Plasmid

#232001

Purpose

Bicistronic expression of CHMP2B Q165X mutant along with an mTagBFP2 transfection marker, connected via 2A sequences.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Expression

Mammalian

Mutation

Q165X

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-3XHA

Plasmid

#232003

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a FLAG-CHMP2B-d55-96

Plasmid

#232013

Purpose

Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

Plasmid

#231998

Purpose

Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B-targeted sgRNA (CHMP2B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only
pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

Plasmid

#222288

Purpose

Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

Depositor

Anne Straube

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Insert

KIF1C (KIF1C Human)

Tags

6xHis, GFP, and GST

Expression

Bacterial

Mutation

stalk region only amino acids 642 to 922

Promoter

T7

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

Plasmid

#222289

Purpose

Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

Depositor

Anne Straube

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Insert

KIF1C (KIF1C Human)

Tags

6His and GST

Expression

Bacterial

Mutation

stalk region only amino acids 642 to 922

Promoter

T7

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)

Plasmid

#212402

Purpose

Encodes SARS-CoV-2 spike L5F,D614G,Q677H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L5F,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L5F,D614G,Q677H

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Rab11 single guide RNA

Plasmid

#229683

Purpose

guide RNA for Rab11 tagging at the N-terminus in pX459

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

Rab11 guide (RAB11A Human)

Use

CRISPR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 G75V (GTA) T76I (ATT)

Plasmid

#212443

Purpose

Encodes SARS-CoV-2 spike G75V, T76I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G75V, T76I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G75V, T76I

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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