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pLV-SV40-puro-EF1a-HA-NLGN1-bc

Plasmid

#200167

Purpose

Expresses HA-tagged human NLGN1

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13900 - pAAV-AiE0458m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3900)

Plasmid

#224040

Purpose

AiE0458m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (strong)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14489 - pAAV-AiE0675m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4489)

Plasmid

#230399

Purpose

AiE0675m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13584 - pAAV-AiE0475m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3584)

Plasmid

#224031

Purpose

AiE0475m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Chandelier cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12858 - pAAV-AiE0675m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2858)

Plasmid

#224014

Purpose

AiE0675m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12385 - pAAV-AiE0548h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2385)

Plasmid

#223990

Purpose

AiE0548h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13084 - pAAV-hDLX-minBG-iCre(R297T)-BGHpA (Alias: CN3084)

Plasmid

#224027

Purpose

hDLX is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical GABAergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14532-pAAV-DLX2.0-minBG-FlpO-WPRE3-BGHpA (Alias: CN4532)

Plasmid

#222939

Purpose

FlpO recombinase expression from the DLX2.0 enhancer

Depositor

Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

FlpO (flp Synthetic)

Use

AAV

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGTM_COV2_NSP5_C145A_001_SUMO

Plasmid

#192349

Purpose

Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

Depositor

Gaetano Montelione

Article

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)

Tags

6X-His-Sumo

Expression

Bacterial

Mutation

Cysteine 145 of the active site is substituted wi…

Promoter

T7 lac

Available Since

June 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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35S::GeNL/pCAMBIA1301

Plasmid

#190070

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Green-enhanced Nano-lantern

Expression

Plant

Promoter

CaMV 35S

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-AP180-GFP

Plasmid

#186577

Purpose

Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP180 (Snap91 Rat)

Tags

FKBP N-terminal GFP C-terminal

Expression

Mammalian

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MANF-Halo

Plasmid

#175341

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MANF (MANF Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NASP-Halo

Plasmid

#175340

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NASP (NASP Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh1

Plasmid

#168984

Purpose

Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-TTc-4xDQNAT

Plasmid

#128401

Purpose

Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK-3xFLAG

Plasmid

#163452

Purpose

lentiviral overexpression vector for human NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET-NtLAtC60αβC20

Plasmid

#160873

Purpose

Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: pBAD, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF39-2xCSTREP

Plasmid

#136200

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF39

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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