Addgene

We narrowed to 16,452 results for: EMB

Showing: 7721 - 7740 of 16452 results

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pCS020

Plasmid

#207915

Purpose

Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKE012

Plasmid

#207914

Purpose

Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKE010

Plasmid

#207910

Purpose

Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS019

Plasmid

#207913

Purpose

Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10D-HA

Plasmid

#209234

Purpose

Mammalian expression of ATP10D

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-Per_sig_pep

Plasmid

#202152

Purpose

Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Peroxisome signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSC-cUnaG

Plasmid

#207631

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-cUnaG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-LPETG

Plasmid

#207629

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-LPETG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP hSmad4-HomDonor

Plasmid

#206044

Purpose

Donor plasmid for CRISPR knock-in

Depositor

Ali Hemmati Brivanlou

Article

Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

Insert

Smad4 (SMAD4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab29

Plasmid

#186021

Purpose

2xHis-Rab29

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-Rab29 (RAB29 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-Venus

Plasmid

#202157

Purpose

Level 0 plasmid containing a Venus fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Venus gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-sfGFP

Plasmid

#202156

Purpose

Level 0 plasmid containing a sfGFP fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sfGFP gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE_mTurquoise2

Plasmid

#202155

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-RFP1

Plasmid

#202154

Purpose

Level 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

RFP1 gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-mRuby3

Plasmid

#202153

Purpose

Level 0 plasmid containing a mRuby3 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRuby3 gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-3xStop

Plasmid

#202151

Purpose

Level 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

3x Stop codon

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-eGFP

Plasmid

#202149

Purpose

Level 0 plasmid containing an eGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eGFP gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-Venus

Plasmid

#202148

Purpose

Level 0 plasmid containing a Venus fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Venus gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_CD-sfGFP

Plasmid

#202147

Purpose

Level 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sfGFP gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-mTurquoise2

Plasmid

#202146

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge