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RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

Plasmid

#192233

Purpose

lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

Plasmid

#192230

Purpose

lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192234

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192229

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192232

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192231

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E,K450R)::flag

Plasmid

#187882

Purpose

Gal4/UAS expression of dSarm(isoE,K450R)::FLAG

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm )

Tags

FLAG

Mutation

K450R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E)::flag

Plasmid

#187871

Purpose

Gal4/UAS expression of dSarm(isoE)::FLAG

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm Fly)

Tags

FLAG

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC

Plasmid

#148442

Purpose

Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCSmChnERT(#99)

Plasmid

#184063

Purpose

cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

Insert

mCherry-nls-ERT2

Use

Synthetic Biology

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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fru-T2A-QF2 HDR plasmid

Plasmid

#141099

Purpose

Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

Depositor

Leslie Vosshall

Article

Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

Insert

fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm

Use

Donor template

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389H

Plasmid

#137658

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389H

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389W

Plasmid

#137657

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389W

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_Nbr

Plasmid

#186662

Purpose

Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-FKBP-HA

Plasmid

#185658

Purpose

Mitochondrial targetted (Cox8) FKBP protein with C-terminal HA epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-FKBP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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ETS2_pLENTI-CAG-IRES-GFP

Plasmid

#177005

Purpose

Mammalian lentiviral expression vector encoding ETS2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ETS2 (ETS2 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Rcan1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176978

Purpose

Mammalian expression vector encoding Rcan1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

Rcan1 (Rcan1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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MIS18A_pcDNA6.2/EmGFP-Bsd

Plasmid

#176973

Purpose

Mammalian expression vector encoding MIS18A and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MIS18A (MIS18A Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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SLC19A1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176951

Purpose

Mammalian expression vector encoding SLC19A1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

SLC19A1 (SLC19A1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX030

Plasmid

#167154

Purpose

MoClo-compatible Level 1 (position 5) vector encoding Neonothopanus nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnCPH-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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