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pMLS285

Plasmid

#73734

Purpose

SapTrap egl-13 NLS connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

egl-13 NLS

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_HHEX_1

Plasmid

#72354

Purpose

Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

HHEX (HHEX Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDual_dsCas9_Blast_hs_TP73

Plasmid

#214691

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73

Depositor

Kevin Janes

Insert

dgRNA_TP73 (TP73 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCN34e-ktrA-yfp

Plasmid

#227640

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AchLightR

Plasmid

#187463

Purpose

Expresses red acetylcholine indicator AchLightR in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightR

Use

AAV

Tags

Igκ leader

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-mGreenLantern

Plasmid

#197922

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern

Expression

Mammalian

Mutation

none

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEY17

Plasmid

#191023

Purpose

flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18 CELE_Y48D7A.2 (flp-18 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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LacZ_pHIVEGFP

Plasmid

#191251

Purpose

Expresses LacZ in mammalian cells (used as a control)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LacZ

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-iGECInano

Plasmid

#186189

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Bacterial

Promoter

AraC

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

His

Expression

Insect

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep-GFP11

Plasmid

#153514

Purpose

TwinStrep-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_His2-GFP11

Plasmid

#153511

Purpose

His2-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

His2-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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JB88

Plasmid

#128933

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RhebSN-BirA*-HA-MAO (RHEB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB87

Plasmid

#128932

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RhebQL-BirA*-HA-MAO (RHEB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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T7-AglC-His6

Plasmid

#89726

Purpose

Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglC

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-PseB-His6

Plasmid

#89723

Purpose

Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseB

Tags

His6

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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