We narrowed to 10,347 results for: plasmids 101

1. Tet-O-FUW-EGFP-P2A-mCherry

   Plasmid

   #203868

   Purpose

   Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GSK3A_pLX307

   Plasmid

   #98341

   Purpose

   Lentiviral expression of GSK3A

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GSK3A (GSK3A Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-pNFkB::Cre-mRuby3

   Plasmid

   #164137

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   NFkB promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   NFkB synthetic promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn1-GRAB-HTR6-cilia

   Plasmid

   #189615

   Purpose

   Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6_GRAB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Syn1

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist-WIV1-CoV Δ18

   Plasmid

   #164439

   Purpose

   Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   WIV1-CoV Spike truncated to remove 18 amino acids
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

   Plasmid

   #224500

   Purpose

   Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FcD265A-FLAG

   Plasmid

   #65420

   Purpose

   Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production

   Depositor

   Dane Wittrup

   Article

   Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

   Insert

   FcD265A-FLAG
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D265A

   Promoter

   Modified CMV Promoter

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMR506-EF1a-H2B-EGFP

   Plasmid

   #186627

   Purpose

   Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

   Depositor

   Jonas Frisén

   Article

   Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

   Insert

   EF1a (EEF1A1 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-pHSE::Cre-mRuby3

   Plasmid

   #164136

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   HSE promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSE synthetic promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

    Plasmid

    #156164

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    THBS1 with addition of ER-retention sequence KDEL

    Promoter

    CMV

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SYKA-c020

    Plasmid

    #175489

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO-Bcl2-IRES-tdtomato(TET005)

    Plasmid

    #117857

    Purpose

    This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    Bcl2 (Bcl2 Mouse)
    

    Use

    Tta/teto

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. UQCRB_pLX307

    Plasmid

    #98381

    Purpose

    Lentiviral expression of UQCRB

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    UQCRB (UQCRB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-VTKS4

    Plasmid

    #170855

    Purpose

    AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ230-RR

    Plasmid

    #108860

    Purpose

    LbCpf1 Gateway entry plasmid with G548R and K611R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G548R and K611R, Cpf1 is rice codon optimized

    Promoter

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-miRFP-6xFLAG

    Plasmid

    #161614

    Purpose

    Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    miRFP-6xFLAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PCBP1_pLX307

    Plasmid

    #98362

    Purpose

    Lentiviral expression of PCBP1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    PCBP1 (PCBP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLHCX-mPM20D1-flag

    Plasmid

    #84566

    Purpose

    mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

    Depositor

    Bruce Spiegelman

    Article

    Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

    Insert

    peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    Retroviral

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-mRuby3-6xFLAG

    Plasmid

    #161613

    Purpose

    Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    mRuby3-6xFLAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330 Rosa26 AscI LH291Gu1 (LH500)

    Plasmid

    #99628

    Purpose

    Plasmid used to target the pre-modified Rosa26 allele with Cas9

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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