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2. p167 Dync1i1.D

Plasmid

#26439

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform D (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
KHBD00744

Plasmid

#39751

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9648 (Max Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00629

Plasmid

#39699

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2189 (Dfd Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00097

Plasmid

#34252

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8933 (exd Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00077

Plasmid

#34235

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3827 (sc Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00751

Plasmid

#39757

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7952 (gt Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pGWB414_35S-(ΔTP)DM3(Hh-0)-HA

Plasmid

#229724

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Hh-0)

Plasmid

#229713

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pGWB414_35S-DM3(Col-0)-HA

Plasmid

#229712

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pSTTa-GNAI1

Plasmid

#248651

Purpose

Vector for protein expression of 2x StrepII-tagged human heterotrimeric G protein GNAI1 in E. coli.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Insert

GNAI1 (GNAI1 Human)

Tags

2x StrepII, Thrombin site, TEV site

Expression

Bacterial

Promoter

tac

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pACEBac1-eIF3i-HIS10

Plasmid

#240032

Purpose

Human eIF3i for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3i (EIF3I Human)

Tags

HIS10

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTARGEX-VAC

Plasmid

#242450

Purpose

Targets high levels of recombinant protein to vacuole. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.

Depositor

Allyson MacLean

Article

Chartrand-Pleau et al Front. Synth. Biol. 3:1619871

Type

Empty backbone

Tags

C-terminal propeptide (CTPP) derived from N. taba…

Expression

Plant

Promoter

double 35S

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW306_U6-sgRNA-EMX1

Plasmid

#244824

Purpose

Mammalian expression of SpyCas9 single guide RNA targeting EMX1

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW124_dCas9

Plasmid

#244821

Purpose

Mammalian expression of catalytically inactive Cas9 (dCas9)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/H840A

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS

Plasmid

#244842

Purpose

Bacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS26_10xHis_MBP_TEV_VRER-Cas9

Plasmid

#244829

Purpose

Bacterial expression of VRER Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

VRER Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135V/G1218R/R1335E/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS16_10xHis_MBP_TEV_xPBA-Cas9

Plasmid

#244830

Purpose

Bacterial expression of xPBA Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

xPBA Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS23_10xHis_MBP_TEV_SpG-Cas9

Plasmid

#244826

Purpose

Bacterial expression of SpG Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS25_10xHis_MBP_TEV_EQR-Cas9

Plasmid

#244828

Purpose

Bacterial expression of EQR Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

EQR Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135E/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(92-168)-EGFP

Plasmid

#196228

Purpose

EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(92-168)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) E83A

Plasmid

#240583

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, E83A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K80A

Plasmid

#240584

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K80A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K97A

Plasmid

#240601

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K97A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K96A

Plasmid

#240585

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K96A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K96A

Plasmid

#240600

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K96A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K97A

Plasmid

#240586

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K97A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K102A

Plasmid

#240588

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K102A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a asyn(66-140) D98A

Plasmid

#240587

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, D98A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn K80A

Plasmid

#240599

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K80A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_Z11 gpN:SpyTag

Plasmid

#225196

Purpose

Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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