We narrowed to 10,026 results for: ADA

1. pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

   Plasmid

   #180313

   Purpose

   bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV and sfCherry2

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-CMV-scFVDnmt3AL_bGHpA

   Plasmid

   #177347

   Purpose

   AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
   

   Use

   AAV

   Tags

   C-terminal domain of mouse Dnmt3l (194–415 aa), P…

   Expression

   Mammalian

   Mutation

   598–908 aa of mouse Dnmt3a

   Promoter

   CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CMV-scFVtet1_bGHpA

   Plasmid

   #177351

   Purpose

   AAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   N terminal domain of human Tet1

   Promoter

   CMV promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-HOPX

   Plasmid

   #192923

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   HOPX (HOPX Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-tdTHC

   Plasmid

   #112621

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-tdTomato (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-Myc-FBW7 Delta F-Box

   Plasmid

   #197452

   Purpose

   The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW7 Fbox deleted.

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-2dV-Camui (T286A)

   Plasmid

   #220367

   Purpose

   T286A phosphodead mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Use

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-VHC

   Plasmid

   #112618

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Venus (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8798 GFP-KrasG12C_2B_hygro

   Plasmid

   #64376

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2B (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   changed glycine 12 to cysteine

   Promoter

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1 KBTBD4 R313PRR

    Plasmid

    #184626

    Purpose

    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 R313PRR expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal Flag tagged KBTBD4 R313PRR (KBTBD4 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    R313PRR

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-NOTCH3(Trunc1)-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157045

    Purpose

    Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT/TO-V5-SHLD3

    Plasmid

    #114124

    Purpose

    Expresses N-terminally tagged V5-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available Since

    Aug. 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 8047_GFP-Kras2B_ires_puro

    Plasmid

    #64371

    Purpose

    This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-hCCND1-T286A-HA

    Plasmid

    #174155

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    A>G at base 856 (Thr>Ala at amino acid 286)

    Promoter

    EF1A

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. DHC1-AID-Clover donor (Neo)

    Plasmid

    #158621

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMX-53BP1(1-1710) 3LA

    Plasmid

    #185698

    Purpose

    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.

    Depositor

    Daniel Durocher

    Article

    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

    Insert

    53BP1(1-1710) 3LA (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Flag tag, HA tag, and tetracysteine tag

    Expression

    Mammalian

    Mutation

    L175A, L517A, and L693A

    Promoter

    SV40

    Available Since

    July 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMX-53BP1(1-1710) 3LA7A

    Plasmid

    #185707

    Purpose

    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.

    Depositor

    Daniel Durocher

    Article

    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

    Insert

    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Flag tag, HA tag, and tetracysteine tag

    Expression

    Mammalian

    Mutation

    T302A, S452A, S523A, S543A, S625A, S784A, and S89…

    Promoter

    SV40

    Available Since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. shERK2b-mlpx-puro

    Plasmid

    #65230

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4-deltaH-AMBRA1-3xFLAG

    Plasmid

    #174158

    Purpose

    Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 1-34

    Promoter

    CMV

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-mTHC

    Plasmid

    #112620

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    mTeal (mTFP1)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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