Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,921 results for: pET

Clear keywords
Showing: 7761 - 7780 of 9921 results

  4. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105806-plasmid-map-sequence-id-207907

    Enlarge

  5. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid
    #105803
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-FLAG; ORF2: TEV-mClover3
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105803-plasmid-map-sequence-id-203399

    Enlarge

  6. T7 Lig-C-HiBiT

    Plasmid
    #215441
    Purpose
    Bacterially expressed T7 Lig-C-HiBit for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Escherichia phage T7 DNA Ligase, C-terminal HiBiT, C-terminal 10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215441-plasmid-map-sequence-id-428723

    Enlarge

  8. N-His-GvpC-ssrA

    Plasmid
    #153297
    Purpose
    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
    Depositor
    Mikhail Shapiro
    Publication
    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d
    Insert
    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    Tags
    His-tag and ssrA tag
    Expression
    Bacterial
    Mutation
    1) N-terminal 6xHis tag flanked by 1x glycine res…
    Promoter
    T7
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153297-plasmid-map-sequence-id-310693

    Enlarge

  9. pRC/CMV-JAK1P1084A-VSV

    Plasmid
    #139358
    Purpose
    Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)
    Depositor
    Sandra Pellegrini
    Publication
    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    Tags
    VSV
    Expression
    Mammalian
    Mutation
    P1084A
    Promoter
    CMV
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    139358-plasmid-map-sequence-id-292222

    Enlarge

  10. pAL950

    Plasmid
    #119748
    Purpose
    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Depositor
    Linda Randall
    Publication
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Tags
    none
    Expression
    Bacterial
    Mutation
    Internal NdeI site removed and mutations Alanine …
    Promoter
    T7
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119748-plasmid-map-sequence-id-232481

    Enlarge

  11. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Publication
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 Mustard Weed, AT2G20180)
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. L3MBTL3 (3UT1)

    Plasmid
    #36901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3 (L3MBTL3 Human)
    Tags
    His tag and Streptavidin-Binding Peptide (SBP)-Ta…
    Expression
    Bacterial
    Mutation
    contains amino acid residues 228-551 only
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pHBT-KRAS174

    Plasmid
    #248050
    Purpose
    To express His6 tag-Avi tag-TEV cleavage site-KRAS (residues 1-174) in E. coli, under the T7 promoter.
    Depositor
    Shohei Koide
    Publication
    Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    KRAS (KRAS Human)
    Tags
    His6 tag-Avi tag-TEV cleavage site
    Expression
    Bacterial
    Mutation
    includes aa 1-174 only
    Promoter
    T7
    Available since
    Nov. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    248050-plasmid-map-sequence-id-493232

    Enlarge

  17. Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L

    Plasmid
    #12474
    Depositor
    David Baker
    Insert
    Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    b19 only. N7K D9T D10V N14T F15Y D16E Y17I Y37L K…
    Available since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only

  19. pJ131

    Plasmid
    #29485
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. pJ145

    Plasmid
    #29491
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 7761 - 7780 of 9921 results