We narrowed to 2,364 results for: NIG

1. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

   Plasmid

   #83485

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R472A_H477A_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFlp-Tc

   Plasmid

   #222336

   Purpose

   Expresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB )
   flp (flp )
   cI (cI )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cl, flp, and sacB

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-c-Met

   Plasmid

   #31786

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS PKC theta

   Plasmid

   #8426

   Depositor

   Frederic Mushinski

   Article

   Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

   Insert

   Mouse Protein Kinase C theta (Prkcq Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 22, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

   Plasmid

   #190928

   Purpose

   Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Tags

   Strep-TEV-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKH3-p190

   Plasmid

   #15547

   Purpose

   Expression of HA-tagged p190 RhoGAP

   Depositor

   Ian Macara

   Article

   Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

   Insert

   p190 GTPase activating protein (Arhgap35 Rat)
   

   Use

   Tags

   Triple HA1 tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pLyGo-Ec-8

   Plasmid

   #163136

   Purpose

   pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P(rhaB)

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTH PKC alpha

   Plasmid

   #8409

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Mouse Protein Kinase C alpha (Prkca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 human A3C

   Plasmid

   #105047

   Purpose

   Overexpression of APOBEC3C in mammalian cell lines

   Depositor

   Carsten Münk

   Article

   Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

   Insert

   APOBEC3C (A3C) (APOBEC3C Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV DCC

    Plasmid

    #16459

    Depositor

    Bert Vogelstein

    Article

    Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

    Insert

    DCC (DCC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-iLIDx6

    Plasmid

    #103779

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    mCherry-iLIDx6
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19 PKC delta

    Plasmid

    #8427

    Depositor

    Frederic Mushinski

    Article

    Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

    Insert

    Mouse Protein Kinase C delta (Prkcd Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-SspBx6

    Plasmid

    #103778

    Purpose

    expression of iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    YFP-SspBx6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLyGo-Kp-1

    Plasmid

    #163143

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-FKBP-GFP-SINTBAD

    Plasmid

    #216840

    Purpose

    Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Lentiviral

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH231-EF1-Puro

    Plasmid

    #115143

    Purpose

    Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Puromycin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. cyto-YFP-FKBPx5

    Plasmid

    #103777

    Purpose

    expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-YFP-FKBPx5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. cyto-CFP-FRBx5

    Plasmid

    #103776

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-CFP-FRBx5
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMP71-mGFP-LLO-mPMEL

    Plasmid

    #174608

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane GFP fused to LLO and mouse PMEL antigen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-OSH2-2xPH

    Plasmid

    #161987

    Purpose

    Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

    Depositor

    David Bryant, Tamas Balla

    Article

    Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

    Insert

    OSH2 (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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