We narrowed to 3,286 results for: golden gate

1. pMA-SpCas9-g7

   Plasmid

   #80790

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEM-T Easy-AtpU6-26

   Plasmid

   #160218

   Purpose

   AtpU6-26 Golden Gate level 0 piece to express gRNAs

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   pAtU6-26 promoter
   

   Use

   Golden gate level 0 piece to express grnas

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMA-SpCas9-g10

   Plasmid

   #80793

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMA-SpCas9-g9

   Plasmid

   #80792

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BB2_EF

   Plasmid

   #98538

   Purpose

   empty BB2 for transcription unit cloning

   Depositor

   Diethard Mattanovich, Brigitte Gasser, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBT100-neo

   Plasmid

   #123137

   Purpose

   Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Neomycin, poly-A terminator
   

   Use

   Synthetic Biology; Golden gate donor vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKEK3230

   Plasmid

   #234102

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   FuGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKEK3236

   Plasmid

   #234108

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   sfCherry2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E117Q/T127I/G219A

   Promoter

   pJ23100-BsaI

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKEK3221

    Plasmid

    #234100

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfcherry 2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E117Q/T127I/G219A

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKEK3237

    Plasmid

    #234091

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKEK3397

    Plasmid

    #234081

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    FuGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKEK3224

    Plasmid

    #234086

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    FuGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKEK3197

    Plasmid

    #234095

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    YukonOFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKEK3199

    Plasmid

    #234097

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    tspurple
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKEK3200

    Plasmid

    #234098

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    fuGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKEK3232

    Plasmid

    #234104

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    tsPurple
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKEK3233

    Plasmid

    #234105

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    YukonOFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKEK3395

    Plasmid

    #234079

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    YukonOFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKEK3400

    Plasmid

    #234084

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge