We narrowed to 848 results for: mcp
-
Plasmid#178483PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)
TagsHis-GSTExpressionBacterialPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD1:TM2-MAR
Plasmid#106214PurposeProvides N.tabacum TM2 matrix attachment region as a level 0 GoldenBraid partDepositorInsertN.tabacum TM2 matrix attachment region
UseSynthetic BiologyExpressionPlantAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment 1
Plasmid#86660PurposeLuciferase reporter containing CDK4 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated from 880 -1 to -213PromoterCDK8Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment A
Plasmid#86658PurposeLuciferase reporter containing CDK4 promoterDepositorPublicationInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the NcoI site from 880 (…PromoterCDK6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Phamret-LaminB1-10
Plasmid#57758PurposeLocalization: Nuclear Envelope, Excitation: 458 / 475, Emission: 458 / 517DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLaminB1 (LMNB1 Human)
TagsPhamretExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-LMNB1
Plasmid#227328PurposeDonor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)DepositorInsertLMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-CEP135
Plasmid#227286PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA Targeting N-terminus of CEP135 (CEP135 Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-CEP135
Plasmid#227287PurposeDonor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-STIL
Plasmid#136844PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLIP-STIL (STIL Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)-strep
Plasmid#180023PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.DepositorInsertGB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-ultraID-LMNB1
Plasmid#207775PurposeDonor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-3xFLAG-LMNB1
Plasmid#207777PurposeDonor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 Homology Arms flanking a Blast-3xFLAG Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-3xHA-LMNB1
Plasmid#207778PurposeDonor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
GB1-PRD(703-868, P801G)-Strep
Plasmid#164444PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to GlycineAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Enhancer-ZC3H12D_PromoterMUT
Plasmid#153068PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE40 binding sites. N…PromoterNoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A30
Plasmid#132052PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A30 (SLC25A30 Human)
ExpressionMammalianAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pHAGE-CDK6-V181I
Plasmid#116218PurposeLentiviral expression of CDK6 V181IDepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
R777-E024 Hs.CDK4-nostop
Plasmid#70308PurposeGateway ORF clone of human CDK4 [NM_000075.3] without stop codon (for C-terminal fusions)DepositorInsertCDK4 (CDK4 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CDK6_SFTA3
Plasmid#205791PurposeExpress mEGFP-tagged fusion protein, CDK6_SFTA3 from patient-derived sequenceDepositorAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only