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pDest17_Gcn4 SATtoG KtoR

Plasmid

#231861

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1-Halo

Plasmid

#224591

Purpose

For expression of FAM177A1-Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

HALO

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

Plasmid

#229797

Purpose

Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

Plasmid

#229793

Purpose

Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED solvvol

Plasmid

#231858

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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ZFPL1-GFP

Plasmid

#224590

Purpose

For expression of ZFPL1-GFP in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

ZFPL1 (ZFPL1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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FAM177A1-SNAP

Plasmid

#224592

Purpose

For expression of FAM177A1-SNAPTag in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

SNAP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16A-I547K-peGFP-N1

Plasmid

#228583

Purpose

Expresses mouse TMEM16A-I547K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

I547K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16A-E551K-peGFP-N1

Plasmid

#228584

Purpose

Expresses mouse TMEM16A-E551K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

E551K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DXO

Plasmid

#177992

Purpose

For gateway cloning of DXO

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

DXO (DXO Human)

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL435

Plasmid

#226628

Purpose

pcDNA-dTdT

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

catalytically deactivated TdT (DNTT Human)

Use

Synthetic Biology

Expression

Mammalian

Mutation

D343E, D345E

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

Plasmid

#194230

Purpose

Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

PGK-LCB3-SynNotch-T2A-mCherry

Use

Lentiviral

Mutation

E45D mutation in LCB3

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideB3GNT5-neo

Plasmid

#220867

Purpose

encodes CRISPR sgRNA targeting human B3GNT5

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

Plasmid

#223163

Purpose

2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pV1210

Plasmid

#242891

Purpose

Modified plasmid from pV1200 to perform ENO1-SI CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Article

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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YY105: pPB_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

Plasmid

#228882

Purpose

PiggyBac plasmid expressing the HaloTag-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3 and PuroR under the control of a UbC promoter.

Depositor

Dingchang Lin

Article

Yan et al bioRxiv [Preprint]. 2025 Jul 18:2025.07.

Insert

HaloTag-fused GEMINI(A)

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-8xGTIIC-mScarlet-NLS-PEST

Plasmid

#249724

Purpose

Nuclear-localized fluorescent reporter of YAP/TAZ activity driven by promoter with TEAD binding sites

Depositor

Tobias Meyer

Article

Ferrick et al bioRxiv [Preprint]. 2024 Nov 4:2024.10.1

Insert

YAP-TEAD responsive promoter (YAP1 Human)

Use

Lentiviral

Tags

mScarlet3-NLS-PEST

Expression

Mammalian

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262V

Plasmid

#234607

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262N

Plasmid

#234608

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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Mito-CanlonicSF/pCMV-myc-mito

Plasmid

#224465

Purpose

To explore mitochondrial lactate dynamics

Depositor

Alejandro San Martín

Article

Rauseo et al bioRxiv [Preprint]. 2024 Jul 20:2024.07.

Insert

CanlonicSF

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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