We narrowed to 14,584 results for: pET

1. Affibody EGFR:1907.BirA*-pET301/NT-DEST

   Plasmid

   #174691

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FabF (KASII) pet28 N-terminal His Tag

   Plasmid

   #75017

   Purpose

   Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

   Depositor

   Michael Burkart

   Article

   Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

   Insert

   FabF (fabF E. coli)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-CTLA4-FLN-ELP-His-ybbr

   Plasmid

   #168038

   Purpose

   Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
   

   Use

   Tags

   3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET mClover LIC cloning vector (H6-mClover)

   Plasmid

   #86931

   Purpose

   pET LIC cloning vector for Biotin-His6-tev-yORF-mClover

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mC…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

   Plasmid

   #167849

   Purpose

   Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10, nsp16
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28b – E. coli RecE residues 564-868

   Plasmid

   #104534

   Purpose

   Expresses E. coli RecE residues 564-868

   Depositor

   Charles Bell

   Article

   Zhang et al Structure. 2009 May 13;17(5):690-702. do

   Insert

   RecE residues 564-866 (C-terminal nuclease domain)
   

   Use

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET mClover LIC cloning vector (u-mClover)

   Plasmid

   #86929

   Purpose

   pET LIC cloning vector for yORF-mClover

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   mClover

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXD8 (human,partial) HIS-tag pET

   Plasmid

   #8569

   Depositor

   Corey Largman

   Insert

   HOXD8 (HOXD8 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Partial protein containing 4 AA N-terminal to HD …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

    Plasmid

    #184407

    Purpose

    yeast surface display of the SARS-CoV-2 Delta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    L452R+T478K

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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