We narrowed to 13,341 results for: GAN

1. mApple-6xHp

   Plasmid

   #214410

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   Spastin aa 43-92 (SPAST Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. NRXN-bio-His

   Plasmid

   #51810

   Purpose

   Expresses full-length Neurexin-1-alpha precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   NRXN (NRXN1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mKate2-P2A-APEX2-TAPP1-PH

   Plasmid

   #67662

   Purpose

   Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-TAPP1-PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaRIgG-2

   Plasmid

   #228996

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-2
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaGIgG-12

   Plasmid

   #228999

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaGIgG-12
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_SC_4.26_STARR-seq

   Plasmid

   #220220

   Purpose

   AAV-STARR-seq screening vector with 4.26 promote

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   4.26

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Neurl1Rm-Flag pcDNA3.1

   Plasmid

   #17319

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Neuralized-like (Neurl1a Mouse)
   

   Use

   RNAi

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pENTR_L1-Turbo-YFP-NLS-STOP-L2

   Plasmid

   #127361

   Purpose

   gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

   Plasmid

   #174890

   Purpose

   Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   U25C PylT

   Promoter

   EF1

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. P2RX1-bio-His

    Plasmid

    #53359

    Purpose

    Expresses full-length P2X purinoceptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    P2RX1 (P2RX1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. HMA-APOBEC1-Nb

    Plasmid

    #226012

    Purpose

    For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

    Depositor

    Eugene Yeo

    Article

    Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    His-MBP and antiRabbit-nanobody

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SELP-bio-His

    Plasmid

    #51639

    Purpose

    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SELP (SELP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-hAR-L26A,F27A

    Plasmid

    #89084

    Purpose

    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR FxxLF mutant (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-L26A,F27A

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pENTR_L1-YFP-miniTurbo-L2

    Plasmid

    #127352

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-hAR-F23A

    Plasmid

    #89089

    Purpose

    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR-FxxLF mutant (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-F23A

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

16. JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

    Plasmid

    #233549

    Purpose

    A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-FAB(ER-LD)

    Plasmid

    #214420

    Purpose

    Detection of ER-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and RspAN-FRB-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAB1679 CMV-HIVNES-GS-Cas13bt3

    Plasmid

    #176317

    Purpose

    CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt3
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_PGK_LTB4R

    Plasmid

    #208087

    Purpose

    Mammalian expression of the wild-type human LTB4 receptor LTB4R.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SERPINE2-bio-His

    Plasmid

    #53416

    Purpose

    Expresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINE2 (SERPINE2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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