We narrowed to 11,713 results for: NSI

1. HIV-1-GFP ΔEnv CA Q67H/N74D

   Plasmid

   #217441

   Purpose

   HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   gag/pol
   

   Use

   Lentiviral

   Mutation

   CA Q67H,N74D

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LCV2 PQBP1 KO1

   Plasmid

   #217447

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   sgRNA targeting PQBP1 (PQBP1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBiFC-SS-VC155-IRE1-dLD

   Plasmid

   #217751

   Purpose

   Expresses the VC fragment of BiFC-IRE1-dLD

   Depositor

   Christina Chan

   Article

   Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

   Insert

   VC155-IRE1-dLD (ERN1 Human)
   

   Tags

   Venus (155-238, A206K)

   Expression

   Mammalian

   Mutation

   deleted amino acids 29-408

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL4.23-WIPI1promotor

   Plasmid

   #206840

   Purpose

   The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   WIPI1 promotor (partial)
   

   Use

   Luciferase

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pINQ-ON10

   Plasmid

   #198689

   Purpose

   Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

   Depositor

   Gualberto González Sapienza

   Article

   Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

   Insert

   anti-SARS-CoV-2 Nucleocapsid nanobody ON10
   

   Tags

   6xHis tag, HA tag, OmpA signal peptide, and Ribos…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pINQ-NbH4

   Plasmid

   #198690

   Purpose

   Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

   Depositor

   Gualberto González Sapienza

   Article

   Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

   Insert

   anti-SARS-CoV-2 Nucleocapsid nanobody H4
   

   Tags

   6xHis tag, Avitag, OmpA signal peptide, and Ribos…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCA528 CHMP4A 153-222

   Plasmid

   #108256

   Purpose

   Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4A residues 153-222 (CHMP4A Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA528 CHMP6 145-201

   Plasmid

   #104597

   Purpose

   Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP6 residues 145-201 (CHMP6 Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA528 CHMP7 366-453

   Plasmid

   #104598

   Purpose

   Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP7 residues 366-453 (CHMP7 Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICH86966::AtU6p::sgRNA:NbCysP6

    Plasmid

    #223217

    Purpose

    Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbCysP6 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Promoter

    Arabidopsis U6 promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCrhaB2_ApyD_ RBS_ApyAOHIS

    Plasmid

    #226247

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-hAT-4_Crp

    Plasmid

    #217168

    Purpose

    Mammalian expression vector for expression of hAT-4_Crp transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    hAT-4_Crp transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-hAT-1_AMi

    Plasmid

    #217170

    Purpose

    Mammalian expression vector for expression of hAT-1_Ami transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    hAT-1_AMi transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Mariner-2_AMi

    Plasmid

    #217171

    Purpose

    Mammalian expression vector for expression of Mariner-2_Ami transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    Mariner-2_AMi transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-hAT-13_AMi

    Plasmid

    #217172

    Purpose

    Mammalian expression vector for expression of hAT-13_Ami transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    hAT-13_AMi transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LT3GEPIR-CCND1shRNA3

    Plasmid

    #220579

    Purpose

    To inducibly knockdown CCND1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    CCND1 shRNA3 (CCND1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNeonGreen-C1-Nir1-631-894

    Plasmid

    #220089

    Purpose

    Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Tags

    NeonGreen-GGSGG

    Expression

    Mammalian

    Mutation

    amino acids 631-894

    Promoter

    CMV

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC1_oROS-HT_LF(C199S)

    Plasmid

    #216412

    Purpose

    Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT_LF(C199S)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC1-lifeact-oROS-HT

    Plasmid

    #216420

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    lifeact-oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNeonGreen-N1-Nir1-613-630

    Plasmid

    #220088

    Purpose

    N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Tags

    GGSGG-NeonGreen

    Expression

    Mammalian

    Mutation

    amino acids 613-630

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge