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pUdOm1.1

Plasmid

#210273

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO2c

Plasmid

#210231

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm7.1

Plasmid

#210287

Purpose

Empty backbone with zeocin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO1k

Plasmid

#210234

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pIL2RmCherry

Plasmid

#105317

Purpose

To visualize membrane dynamics

Depositor

Kenneth Yamada

Article

Artym et al Eur J Cell Biol. 2011 Feb-Mar;90(2-3):17

Insert

mCherry

Expression

Mammalian

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_GFP

Plasmid

#221469

Purpose

Gateway cloning of GFP overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Gateway Cloning

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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RNF126

Plasmid

#138649

Purpose

Mammalian expression of full-length wild-type RNF126

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-VAV1

Plasmid

#242833

Purpose

Encodes human VAV1 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pIAGO-amphDI

Plasmid

#114459

Purpose

Expression of AmphDI glycosyltansferase in streptomycetes

Depositor

Patrick Caffrey

Article

Stephens et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

amphDI

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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U_(pos36GFP)-Cys

Plasmid

#71758

Purpose

U_+36GFP-Cys fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_+36GFP-Cys

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLX307_CdTag-HA_EmptyVector

Plasmid

#221467

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTSAR3.1t

Plasmid

#210254

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

mCerulean
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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CMV plasmid

Plasmid

#169739

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA -TMEM9B-3xHA

Plasmid

#225509

Purpose

TMEM9B expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM9B (TMEM9B Human)

Tags

3xHA

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2-FLAG

Plasmid

#91944

Purpose

Bacterial expression of maize SAE1 and SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-UVRAG S498A

Plasmid

#86744

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

mCherry

Expression

Mammalian

Mutation

S498A

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-UVRAG S498A

Plasmid

#86741

Purpose

N-terminal EGFP-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

EGFP

Expression

Mammalian

Mutation

S498A

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGST2-Bro1 (1-387)

Plasmid

#17635

Depositor

James Hurley

Article

Kim et al Dev Cell. 2005 Jun . 8(6):937-47.

Insert

Bro1 (1-387) (BRO1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-DNM2-W525L

Plasmid

#155263

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Tags

HA

Expression

Mammalian

Mutation

Tryptophan 525 to Leucine

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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