We narrowed to 11,284 results for: VARS

1. MSCV-N BARF1

   Plasmid

   #37922

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   BARF1 (BARF1 H. Herpesvirus 4 (EBV))
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pαH-S-GSAS-OMICRON.BA.1-RBD

   Plasmid

   #184831

   Purpose

   Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6X His tag

   Expression

   Mammalian

   Mutation

   SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_CXCR4_WT

   Plasmid

   #81957

   Purpose

   Gateway Donor vector containing CXCR4, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

   Plasmid

   #114427

   Purpose

   Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GFP, tTA2
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Promoter

   PGK, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PIG-MycT58A

   Plasmid

   #177648

   Purpose

   Expression of mouse Myc with T58A point mutation

   Depositor

   Trudy Oliver

   Article

   Mollaoglu et al Cancer Cell. 2017 Feb 13;31(2):270-285.

   Insert

   Myc-T58A (Myc Mouse)
   

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   T58A point mutation

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ai133 (TITL-ssAPEX2tm) targeting vector

   Plasmid

   #114432

   Purpose

   Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   ssAPEX2tm
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   TREtight

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR D-TOPO-C3_33

   Plasmid

   #60275

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   GLIS3 enhancer (GLIS3 Human)
   

   Use

   Entry vector

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

   Plasmid

   #114081

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.1

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_AR_WT

   Plasmid

   #82094

   Purpose

   Gateway Donor vector containing AR , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   AR (AR Human)
   

   Use

   Gateway entry vector

   Mutation

   p.81_82insQQ; deletion of AA397-482

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_ERBB2_WT

    Plasmid

    #82300

    Purpose

    Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-eGFP-SHLD2-m1

    Plasmid

    #114120

    Purpose

    Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (m1 mutant) (SHLD2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    W489A, F494A, W495A mutations introduced

    Promoter

    TRE promoter, Tet ON

    Available Since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR223_MAPK3_WT

    Plasmid

    #82313

    Purpose

    Gateway Donor vector containing MAPK3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Gateway entry vector

    Mutation

    L180X; p.135_136delSQ-insRPAAE; p.138_379del

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_CBX1_WT

    Plasmid

    #82102

    Purpose

    Gateway Donor vector containing CBX1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CBX1 (CBX1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-N BALF1

    Plasmid

    #37921

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BALF1 (BALF1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223_HRAS_p.Q61K

    Plasmid

    #81319

    Purpose

    Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry vector

    Mutation

    Q61K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid

    #170228

    Purpose

    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    Fusion

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid

    #114093

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548V and N552R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-KrasWT/sgKras/Cre

    Plasmid

    #99848

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_KRAS_p.A59G

    Plasmid

    #81662

    Purpose

    Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry vector

    Mutation

    A59G

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-N E4orf3

    Plasmid

    #38061

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    E4 orf3
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only