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pME_G_0_1-2_005_OC5'5

Plasmid

#235805

Purpose

5' Operon Connector 5 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 5

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1-2_006_OC5'6

Plasmid

#235806

Purpose

5' Operon Connector 6 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 6

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_017_5C7SF

Plasmid

#235795

Purpose

5' Connector 7 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 7 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_006_5C1SR

Plasmid

#235796

Purpose

5' Connector 1 Short Reverse in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 1 Short Reverse

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_009_5C2SR

Plasmid

#235797

Purpose

5' Connector 2 Short Reverse in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 2 Short Reverse

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_019_5C7SR

Plasmid

#235800

Purpose

5' Connector 7 Short Reverse in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 7 Short Reverse

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1-2_001_OC5'1

Plasmid

#235801

Purpose

5' Operon Connector 1 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 1

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_008_5C2SF

Plasmid

#235790

Purpose

5' Connector 2 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 2 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_011_5C3SF

Plasmid

#235791

Purpose

5' Connector 3 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 3 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_022_5C4SF

Plasmid

#235792

Purpose

5' Connector 4 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 4 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_014_5C5SF

Plasmid

#235793

Purpose

5' Connector 5 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 5 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_005_5C1SF

Plasmid

#235789

Purpose

5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 1 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_3b_029_mScarlet-I

Plasmid

#235882

Purpose

mScarlet-I N-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mScarlet-I N-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-AtmiR173aTS-B/c

Plasmid

#227964

Purpose

Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtmiR173aTS-B/c

Plasmid

#227965

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Expression

Plant

Promoter

35S and None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-NbmiR482aTS-B/c

Plasmid

#227966

Purpose

Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
NbmiR482aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_HS∆P2

Plasmid

#225197

Purpose

Used to create a markerless deletion of the E. coli HS P2 phage

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

P2 phage homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge