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pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pTC362

Plasmid

#91216

Purpose

protoplast vector for targeted deletion of 6 genes in tomato

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting 6 tomato genes

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-cdh5 TAL2

Plasmid

#41834

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

cadherin 5 (cdh5 Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAP1791-1

Plasmid

#105246

Purpose

human eGFP with homology arms for RAB11A (recoded )

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for RAB11A (recoded *) (RAB11A Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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LCV2 V-2

Plasmid

#84805

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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AI-CN590

Plasmid

#62596

Purpose

KCTD13 N-terminus disruption by homolgy-directed repair

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Insert

Cerulean-P2A-PuroR (KCTD13 Human)

Use

TALEN; Homology-directed repair donor

Expression

Mammalian

Promoter

hEF1alpha

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSC3

Plasmid

#91181

Purpose

T-DNA vector for targeted deletion of 58kb region in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting 58kb region in medicago truncatula

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRCIscript-crhr2_TAL1A

Plasmid

#44236

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1A

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRCIscript-crhr2_TAL1B

Plasmid

#44237

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1B

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-cdh5 TAL1

Plasmid

#41833

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

cadherin 5 (cdh5 Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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4932 TRV2-TnpB2xSV40nls-HHreRNA4PDS

Plasmid

#251821

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

2xSV40nls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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4927 TRV2-TnpB-reRNA4PDS

Plasmid

#251814

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS with HDV at 3' end

Tags

AtFDnls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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4931 TRV2-TnpB2xSV40nls-reRNA4PDS

Plasmid

#251820

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS with HDV at 3' end

Tags

2xSV40nls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_19 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-NLS-HbaUTR

Plasmid

#252015

Purpose

In vitro transcription of Cas9 only for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_18 - pCMV-Cas9-BLM(1-193)

Plasmid

#252014

Purpose

Mammalian expression of Cas9-BLM(2-194) TruEditor

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BLM(2-194)

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_17 - pLenti_EFS-Cas9-BARD1(32-117)-2A-BlastR

Plasmid

#252013

Purpose

Lentiviral mammalian expression of Cas9-BARD1(32-117) TruEditor

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BARD1(32-117)

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_16 - pLenti_EFS-Cas9-BLM(1-193)-2A-BlastR

Plasmid

#252012

Purpose

Lentiviral mammalian expression of Cas9-BLM(2-194) TruEditor

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BLM(2-194)

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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