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We narrowed to 8,325 results for: gnal

Showing: 7901 - 7920 of 8325 results

  1. OPRK1-DuET

    Plasmid
    #213359
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRK1 (OPRK1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213359-plasmid-map-sequence-id-430881

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  5. pUAS-Myc-BirA-G3-ER

    Plasmid
    #173815
    Purpose
    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site
    Depositor
    Norbert Perrimon
    Article
    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:
    Insert
    Myc-BirA-G3-ER
    Use
    Tags
    Myc
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    173815-plasmid-map-sequence-id-340316

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  6. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Article
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Use
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

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  7. HTR1D-DuET

    Plasmid
    #213318
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1D (HTR1D Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213318-plasmid-map-sequence-id-430855

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  8. dCas9-NLS-gs10-cLYN268

    Plasmid
    #188259
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cLYN268 (LYN Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    188259-plasmid-map-sequence-id-374580

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  9. HTR1B-DuET

    Plasmid
    #213317
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1B (HTR1B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213317-plasmid-map-sequence-id-430854

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  11. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162680-plasmid-map-sequence-id-321455

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  13. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid
    #120506
    Purpose
    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC H84NLS (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of nuclear localization signal sequence
    Promoter
    EF1a
    Available Since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    120506-plasmid-map-sequence-id-234926

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  14. pMIG-hNFATc2/C - AVITEV

    Plasmid
    #74058
    Purpose
    retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74058-plasmid-map-sequence-id-142261

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  16. GABBR1-DuET

    Plasmid
    #213247
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GABBR1 (GABBR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213247-plasmid-map-sequence-id-430899

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  17. CALCRb-DuET

    Plasmid
    #213193
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CALCRb (CALCRL Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213193-plasmid-map-sequence-id-430758

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  18. pAAV-EGFP.T2A.NCLX

    Plasmid
    #181873
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    Use
    AAV and Adenoviral
    Tags
    HA and myc
    Expression
    Mammalian
    Mutation
    Promoter
    synthetic hybrid CAG promoter and synthetic hybri…
    Available Since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181873-plasmid-map-sequence-id-355952

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  20. ADRB2-DuET

    Plasmid
    #213181
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213181-plasmid-map-sequence-id-430748

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Showing: 7901 - 7920 of 8325 results