We narrowed to 15,732 results for: LEA

1. pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

   Plasmid

   #231973

   Purpose

   Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
   

   Use

   Lentiviral

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-Tet-On-HTR6-RhoA sensor

   Plasmid

   #189614

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6 (HTR6 Human)
   

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Promoter

   Tet3G

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3Basic_ME.1/ApoEpromoter

   Plasmid

   #51435

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   ME.1 enhancer is fused upstream of ApoE promoter …

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

   Plasmid

   #208958

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PEA1-Puro-HEK3 CTT ins

   Plasmid

   #171996

   Purpose

   Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-Tau-cYFP

   Plasmid

   #92205

   Purpose

   Retroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   MAPT (MAPT Human)
   

   Use

   Retroviral

   Tags

   YFP(156-239)

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

7. TRIPZ-bioGEF-SUMO1nc-PURO

   Plasmid

   #208042

   Purpose

   Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   SUMO1nc (SUMO1 Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Mutation

   Q94P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATX3-77Q

   Plasmid

   #184249

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Codon optimization for protein expression in BL2…

   Promoter

   T7 promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Halo-Tag-128-425-Akap9

   Plasmid

   #196873

   Purpose

   Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Halo-Tag-Akap9 (128-425) (Akap9 Rat)
   

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLED.GluA2

    Plasmid

    #193017

    Purpose

    GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti GW V5 Eco/Dam hum LaminA

    Plasmid

    #182673

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

    Depositor

    Bas van Steensel

    Article

    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Promoter

    HSP

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

    Plasmid

    #196885

    Purpose

    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-Tau-nYFP

    Plasmid

    #92204

    Purpose

    Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Retroviral

    Tags

    YFP(1-155)

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLED.ENS

    Plasmid

    #193016

    Purpose

    Excitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBactin-td-mCherry-Cdk5rap2-CTD

    Plasmid

    #196854

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)

    Plasmid

    #217805

    Purpose

    Unfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112214

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Mutation

    D10A,H840A,N863A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid

    #73209

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Human, Synthetic)
    

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218910

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
    

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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