We narrowed to 14,384 results for: nts

1. pcDNA5/FRT/TO - RBM28 E8 G>T minigene

   Plasmid

   #169272

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E8 G>T minigene (RBM28 Human)
   

   Expression

   Mammalian

   Mutation

   NM_018077.2:c.(946G>T)

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

   Plasmid

   #169273

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 Exon1-6 WT minigene (RBM28 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

   Plasmid

   #169274

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 Exon1-6 GT>A minigene (RBM28 Human)
   

   Expression

   Mammalian

   Mutation

   NM_018077.2:c.(541+1_541+2delinsA)

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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4. M51 Super 8x FOPFlash (TOPFlash mutant)

   Plasmid

   #12457

   Purpose

   Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   mutant TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. p300dAIL KAT LIC 1M

   Plasmid

   #233588

   Purpose

   Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300dAIL KAT (EP300 Human)
   

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1523-1554

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. FSW-HRP-V5-LRRTM2

   Plasmid

   #82537

   Purpose

   Lentiviral vector for expressing HRP-LRRTM2 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   LRRTM2 (LRRTM2 Human)
   

   Use

   Lentiviral

   Tags

   HRP and V5

   Expression

   Mammalian

   Promoter

   Human Synapsin

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-lyso-pHluorin

   Plasmid

   #70113

   Purpose

   Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHluorin (Cd63 Aequorea victoria, Mouse)
   

   Tags

   HA-Tag and pHluorin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-myc-HLA-A24

   Plasmid

   #135507

   Purpose

   Mammalian expression of myc-tagged HLA-A*024:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Expression

    Mammalian

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid

    #233036

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiBiT-FZD7

    Plasmid

    #195845

    Purpose

    Expresses human FZD7 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD7 (FZD7 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HsB2AR-mCherry

    Plasmid

    #137785

    Purpose

    Visualization of the Beta-2-adrenergic receptor

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human beta2-adrenergic receptor (ADRB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUW-TetO-ZEB2

    Plasmid

    #207203

    Purpose

    doxycycline-inducible expression of Human ZEB in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-ZEB2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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