We narrowed to 42,759 results for: Ina

1. MSP7-bio

   Plasmid

   #47735

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP7
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. HcRed-pcw107-V5

   Plasmid

   #64647

   Purpose

   (control) when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   n/a
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   PGK

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CAG-eYFP-3x-miR708-5p-TS

   Plasmid

   #117381

   Purpose

   An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sema3f(L,+)-AP-His

   Plasmid

   #72021

   Purpose

   Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3f (Sema3f Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC175

   Plasmid

   #118890

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mGFP, cleav…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSP5-bio

   Plasmid

   #47718

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP5 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP5
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LAP2-beta-actin in modified pEGFP

   Plasmid

   #34839

   Depositor

   Alice Ting

   Article

   Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

   Insert

   LAP2-beta-Actin (ACTB Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

8. SI16-Kit a

   Plasmid

   #58948

   Purpose

   expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

   Depositor

   Gavin Wright

   Article

   Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

   Insert

   Kit receptor a mouse monoclonal antibody (kita Zebrafish)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGEX nsp1 R99A CoV2

   Plasmid

   #175515

   Purpose

   For protein expression of R99A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   R99A nsp1 CoV2
   

   Tags

   GST tag

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. RhopH3-bio

    Plasmid

    #47781

    Purpose

    Expresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RhopH3
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L-YARS2-9

    Plasmid

    #166533

    Purpose

    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOCC120

    Plasmid

    #118892

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mGFP, HIS6, c…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFPm-DAD M1041A

    Plasmid

    #25411

    Depositor

    Arthur Alberts

    Article

    Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

    Insert

    Diap3 (Diaph3 Mouse)
    

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Alanine substitution at critical residue in DAD t…

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLXSN-FLK1 TM

    Plasmid

    #65249

    Purpose

    Expression of dominant negative FLK1 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

    Insert

    FLK1 TM (Kdr Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    deleted AA 786-1346

    Promoter

    LTR

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-Strep/HA-UBE2QL1

    Plasmid

    #124665

    Purpose

    Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sema4f.b-Fc-His

    Plasmid

    #72159

    Purpose

    Expresses the extracellular region of the Sema4F, isoform b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4f.b (Sema4f Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUAST-SIK3 K70M

    Plasmid

    #52973

    Purpose

    insect expression of SIK3 K70M (kinase dead mutant)

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    K70M kinase dead mutant

    Promoter

    Hsp70

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. O-MARS-11

    Plasmid

    #166541

    Purpose

    scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sema5a-AP-His

    Plasmid

    #72035

    Purpose

    Expresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema5a (Sema5a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge