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Plasmid#168496PurposeMammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus.DepositorInsertN-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E11: attB5r-RU5u-His6-nostop-attB1r
Plasmid#162953PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E25: attB4-mPol2p>-attB5
Plasmid#162981PurposeGateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r
Plasmid#162956PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHis6-Halotag fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r
Plasmid#162935PurposeGateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoterDepositorInsertMaltose-binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E09: attB4-Grp78p>-attB5
Plasmid#162974PurposeGateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E09: attB5r-RU5u-FLAG-nostop-attB1r
Plasmid#162951PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertFLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E59: attB5r-RU5u-2xHA-nostop-attB1r
Plasmid#162966PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteinsDepositorInsertDual HA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E42: attB5r-RU5u-Strep2-nostop-attB1r
Plasmid#162961PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteinsDepositorInsertStrep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E26: attB5r-RU5u-myc-nostop-attB1r
Plasmid#162960PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteinsDepositorInsertMyc epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E10: attB5r-RU5u-HA-nostop-attB1r
Plasmid#162952PurposeGateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteinsDepositorInsertHA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E11: attB4-Grp78p>-attB1r
Plasmid#162970PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E10: attB4-FerHp>-attB1r
Plasmid#162969PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E24: attB5r-RU5u-V5-nostop-attB1r
Plasmid#162959PurposeGateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteinsDepositorInsertV5 epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E34: attB5r-iRFP670-nostop-attB1r
Plasmid#162947PurposeGateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E01: attB4-polyhedrinp>His6-nostop-attB1r
Plasmid#162932PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion with AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C415-E01: attB4-stuffer-attB1r
Plasmid#162931PurposeGateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectorsDepositorInsertstuffer fragment for vector controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOCT4-Cyan-pac
Plasmid#160871PurposeMarmoset TargetingDepositorInsertmCerulean3
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A-GFP
Plasmid#166837PurposeExpresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationG2APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#471
Plasmid#110935PurposeExpresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tailDepositorInsertsmig-13 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only