We narrowed to 22,792 results for: promoter

1. pLKO-Tet-On shYAP1-2

   Plasmid

   #193665

   Purpose

   Tet inducible knockdown of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-EGFP MASTL E167D (siRNA resistant)

   Plasmid

   #191013

   Purpose

   Expresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E167D

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Coexpressed Free ClLIS1 (M)

   Plasmid

   #182484

   Purpose

   Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS(M)
   LS
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Coexpressed Free deadFPPS-NES

   Plasmid

   #182492

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   deadFPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. IL2-mCherry-AH in pmCherryN1

   Plasmid

   #187284

   Purpose

   Express IL2-AH-mCherry

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH

   Promoter

   CMV

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL2-mCherry-AHA740D, E758K in pmCherryN1

   Plasmid

   #187285

   Purpose

   Express IL2-AH A740D, E758K-mCherry

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH A740D, E758K

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-hFAM3B-HA

   Plasmid

   #180145

   Purpose

   Coding sequence of human FAM3B pCS2 with C terminal HA tag

   Depositor

   Edward De Robertis

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   FAM3B (FAM3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-2T-TRX-C751-E3616S

   Plasmid

   #182844

   Purpose

   mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRX (w Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166133

   Purpose

   This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting cldn1 gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166134

    Purpose

    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HEpl016

    Plasmid

    #179991

    Purpose

    pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    SiCas12i
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-hFAM3B-FLAG

    Plasmid

    #180146

    Purpose

    Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

    Depositor

    Edward De Robertis

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Insert

    FAM3B (FAM3B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

    Plasmid

    #172208

    Purpose

    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL

    Plasmid

    #172205

    Purpose

    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-DEST42-dre4

    Plasmid

    #170440

    Purpose

    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    dre4 (dre4 Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-20

    Promoter

    T7

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMC00008

    Plasmid

    #166724

    Purpose

    sgRNA cloning backbone

    Depositor

    Raj Chari

    Article

    Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti_CRISPRi_sgAR10

    Plasmid

    #167003

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 10 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. KLC1N343S-Myc

    Plasmid

    #166963

    Purpose

    Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1-N343S (Klc1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    KLC1-N343S

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB.His.MBP.3C NLRP1 CARD G1467C

    Plasmid

    #164009

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467C

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    G1467C

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB.His.MBP.3C NLRP1 UPA-CARD E1461R

    Plasmid

    #164007

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    E1461R

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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