Addgene

Showing: 61 - 80 of 8428 results

Results per page:

Sort results by:

pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

Plasmid

#47904

Purpose

An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

eNpHR3.0

Use

AAV

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC337 - pAAV EF1a DIO mCherry

Plasmid

#47636

Purpose

An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC589 - pAAV c-fos Nuc-eYFP

Plasmid

#59133

Purpose

An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

mFos

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

Plasmid

#125146

Purpose

An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

Depositor

Christopher Richie

Insert

HA-tagged hM4D(Gi) (CHRM4 Human)

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

SYN1

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq)

Plasmid

#125147

Purpose

An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM3D(Gq)) from a synapsin promoter

Depositor

Christopher Richie

Insert

HA-tagged hM3D(Gq) (CHRM3 Human)

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

SYN1

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

Plasmid

#112719

Purpose

An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Use

AAV

Tags

Expression

Mutation

D10A

Promoter

Mecp2

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

mFos

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC446 - pAAV EF1a hPREP(S554A)

Plasmid

#59968

Purpose

An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

S554A

Promoter

EF1a

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1553 - pAAV EF1a EGFP-KASH

Plasmid

#131682

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Insert

EGFP-KASH

Use

AAV

Tags

KASH

Expression

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

Plasmid

#75295

Purpose

An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

Depositor

Brandon Harvey

Insert

eGFP-2A-mKv1.2 (Kcna2 Mouse, Synthetic)

Use

AAV

Tags

Expression

Mutation

Promoter

human SYN1

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Ankyrin-G (blotting) [N106/20R]

Plasmid

#177495

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (blotting) (Human). Derived from hybridoma N106/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ankyrin-G (blotting) (Homo sapiens) recombinant mouse monoclonal antibody (ANK3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTre-Tight-luciferase-Hottip (522)

Plasmid

#65490

Purpose

positive control for lncRNA expression assay

Depositor

Rory Johnson, Roderic Guigo

Insert

Hottip (HOTTIP Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-P70a-Top-T~P70a-Bottom-T

Plasmid

#87314

Purpose

Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1617 pAAV SYN1 Nuc-EYFP

Plasmid

#135567

Purpose

An AAV vector expressing a neuronally expressed nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

empty
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

hSYN1

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOttc1494 - pAAV CaMKII SERCaMP-No Tag

Plasmid

#192601

Purpose

An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

Depositor

Brandon Harvey

Insert

SERCaMP

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH229-Tier1-OTtgO2-PCMVmin2-SEAP

Plasmid

#169565

Purpose

Tier-1 vector encoding PTtgO2-driven SEAP expression (OTtgO2-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

phloretin-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TtgO2-PCMVmin

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1079 - pAAV TH gRNA A EF1a EGFP

Plasmid

#113159

Purpose

An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

Plasmid

#47631

Purpose

An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

eNpHR3.0

Use

AAV and Cre/Lox

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

Plasmid

#112683

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-iCre and Nuclear localization signal

Expression

Mutation

Promoter

CMV-IE

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOttc438 - pAAV EF1a CRE ERT2 bGHpA

Plasmid

#210415

Purpose

AAV viral vector packaging plasmid that expresses tamoxifen-regulated Cre recombinase

Depositor

Brandon Harvey, Christopher Richie

Insert

Cre-ERT2

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge